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AlphaLISA SureFire Ultra Human Phospho-AKT1 (Ser473) Detection Kit, 500 Assay Points
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AlphaLISA SureFire Ultra Human Phospho-AKT1 (Ser473) Detection Kit, 500 Assay Points
The AlphaLISA™ SureFire® Ultra™ phospho-AKT1 (Ser473) assay kit can be used to measure levels of AKT1 phosphorylated at residue Ser473 in cellular lysates using no-wash Alpha technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Sample Volume | 10 µL |
The AlphaLISA™ SureFire® Ultra™ phospho-AKT1 (Ser473) assay kit can be used to measure levels of AKT1 phosphorylated at residue Ser473 in cellular lysates using no-wash Alpha technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Human Phospho-AKT1 (Ser473) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Phospho-AKT1 (Ser473) Detection Kit, 500 Assay Points
Product information
Overview
Formats:
- The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
- The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
- The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
- The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.
In the AlphaLISA™ SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.
AlphaLISA™ SureFire® Ultra™ kits are compatible with:
- Cell and tissue lysates
- Antibody modulators
- Biotherapeutic antibodies
Alpha SureFire® kits can be used for:
- Cellular kinase assays
- Receptor activation studies
- Screening
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Cellular or Signaling Pathway |
PI3K/Akt/mTOR
|
| Cellular Process |
PI3K/Akt/mTOR
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
Akt1
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Cardiovascular
Central Nervous System
Metabolic
|
| Unit Size |
500 Assay Points
|
Image gallery
AlphaLISA SureFire Ultra Human Phospho-AKT1 (Ser473) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Phospho-AKT1 (Ser473) Detection Kit, 500 Assay Points
Video gallery
AlphaLISA SureFire Ultra Human Phospho-AKT1 (Ser473) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Phospho-AKT1 (Ser473) Detection Kit, 500 Assay Points
Resources
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