AKT, also known as protein kinase B, is a crucial regulator of several physiological functions, including as transcription, apoptosis, glucose metabolism, and cell survival. It has been demonstrated that AKT's three closely related isoforms-AKT1, AKT2, and AKT3-act on both common and unusual downstream targets. Many triggers can cause AKT to become activated, at which point it is delivered to the membrane to be phosphorylated. AKT phosphorylation of Thr308 by PDK1 and Ser473 by mTORC2 initiates activation. Then AKT separates from the membrane and phosphorylates targets in the cell nucleus and cytoplasm. Deregulation of AKT has been linked with conditions such as diabetes, heart disease, and cancer.

The AlphaLISA SureFire Ultra Human and Mouse Phospho-AKT1 (Ser129) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-AKT1 (Ser129) in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening