The AlphaLISA™ SureFire® Ultra™ Human and Mouse Phospho-AKT1 (Ser129) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1 (Ser129) in cellular lysates using Alpha Technology.
For research use only. Not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Cell Signaling |
Sample Volume | 10 µL |
The AlphaLISA™ SureFire® Ultra™ Human and Mouse Phospho-AKT1 (Ser129) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1 (Ser129) in cellular lysates using Alpha Technology.
For research use only. Not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AKT, also known as protein kinase B, is a crucial regulator of several physiological functions, including as transcription, apoptosis, glucose metabolism, and cell survival. It has been demonstrated that AKT's three closely related isoforms-AKT1, AKT2, and AKT3-act on both common and unusual downstream targets. Many triggers can cause AKT to become activated, at which point it is delivered to the membrane to be phosphorylated. AKT phosphorylation of Thr308 by PDK1 and Ser473 by mTORC2 initiates activation. Then AKT separates from the membrane and phosphorylates targets in the cell nucleus and cytoplasm. Deregulation of AKT has been linked with conditions such as diabetes, heart disease, and cancer.
The AlphaLISA SureFire Ultra Human and Mouse Phospho-AKT1 (Ser129) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-AKT1 (Ser129) in cellular lysates, using Alpha Technology.
Application |
Cell Signaling
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Modality |
Alpha
|
Lysis Buffer Compatibility |
Lysis Buffer
|
Molecular Modification |
Phosphorylation
|
Product Group |
Kit
|
Sample Volume |
10 µL
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
AKT1
|
Target Class |
Phosphoproteins
|
Target Species |
Human
Mouse
|
Technology |
Alpha
|
Therapeutic Area |
Oncology
|
Unit Size |
500 Assay Points
|
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