AIOLOS, also known as Ikaros family zinc finger protein 3, is a transcription factor encoded by the IKZF3 gene. Three members of this protein family (IKAROS, AIOLOS and HELIOS) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. Overexpression of Ikaros protein family members is associated with uncontrolled proliferation and survival of cancer cells and is associated with cancers such as malignant plasma cells and multiple myeloma.

The AlphaLISA SureFire Ultra Human Total AIOLOS Detection Kit is a sandwich immunoassay for the quantitative detection of total AIOLOS in cellular lysates, using Alpha Technology.

 Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening