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AlphaLISA SureFire Ultra Human & Mouse Phospho-4EBP1 (Thr37/46) Detection Kit, 100 Assay Points
The AlphaLISA™ SureFire® Ultra™ p-4E-BP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product information
Overview
The AlphaLISA™ SureFire® Ultra™ p-4E-BP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology.
In the AlphaLISA™ SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.
AlphaLISA™ SureFire® Ultra™ kits are compatible with:
- Cell and tissue lysates
- Antibody modulators
- Biotherapeutic antibodies
Alpha SureFire® kits can be used for:
- Cellular kinase assays
- Receptor activation studies
- Screening
Specifications
| Assay Pathway |
PI3K/Akt/mTOR
|
|---|---|
| Assay Points |
100
|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Method |
Alpha
|
| Experimental Type |
In vitro
|
| Format |
Microplates
|
| One Unit Contains |
100.0 assay points
|
| Quantity in a Package Amount |
1.0 per pack
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Therapeutic Area |
Metabolic
|
| Unit Size |
100 assay points
|
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Resources
Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...
SDS, COAs, Manuals and more
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LanguageEnglishCountryUnited States
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LanguageEnglishCountryEU
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Lot numberU17541Lot dateApril 19, 2024
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Lot numberU18256Lot dateApril 19, 2024
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