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Deoxythymidine 5'-Triphosphate, [Methyl-3H]-, Tetrasodium Salt, Specific Activity: 70-90 Ci (2.59-3.33 TBq)/mmol, 250 µCi (9.25 MBq)

dTTP, tritiated on the methyl group. This product is supplied in aqueous solution. dTTP that is tritiated on the methyl ground will incorporate labeled thymidine into DNA via DNA polymerase.

For research use only. Not for use in diagnostic procedures.

Feature Specification
Concentration 2.5 mCi/ml
Label Position Specifically Labeled
Molecular Weight 570.6

dTTP, tritiated on the methyl group. This product is supplied in aqueous solution. dTTP that is tritiated on the methyl ground will incorporate labeled thymidine into DNA via DNA polymerase.

For research use only. Not for use in diagnostic procedures.

Product Variants
Unit Size: 250 µCi
Part #:
NET221A250UC
List Price
USD 951.73
Unit Size: 1 mCi
Part #:
NET221A001MC
List Price
USD 1,971.00

Overview

250 µCi quantity of Deoxythymidine 5'-Triphosphate, [Methyl-3H]-, Aqueous Sol, Tetrasodium Salt, Specific Activity: 70-90 Ci is available for your research. Deoxythymidine triphosphate (dTTP), labeled on the methyl group with 3H. Tritiated dTTP is commonly used to make probes for in situ hybridization studies via nick translation, or to measure nucleotide incorporation via reverse transcriptase or DNA polymerase activity.

References:

  1. Fonatsch, C., Gradl, G., Kolbus, U. et al. Hum Genet (1990) Chromosomal in situ hybridization of a Hodgkin's disease-derived cell line (L540) using DNA probes for TCRA, TCRB, MET, and rRNA, Human Genetics, 84:427.
  2. White, E.L., Parker, W.B., Macy, L.J., Shaddix, S.C., McCaleb, G., Secrist, J.A., Vince, R., Shannon, W.M. (1989) Comparison of the effect of Carbovir, AZT, and dideoxynucleoside triphosphates on the activity of human immunodeficiency virus reverse transcriptase and selected human polymerases, Biochemical and Biophysical Research Communications, 161(2):393-398.

Specifications

Concentration
2.5 mCi/ml
Molecular Weight
570.6
Application
Drug Discovery & Development
Brand
NEN Radiochemicals
Buffer/Solvent
Aqueous solution in 10mM tricine-NaOH buffer
Detection Modality
Radiometric
Label Position
Specifically Labeled
Nucleotide
dTTP
Packaging
NENSure vial
Radioisotope
3H
Shipping Conditions
Shipped in Dry Ice
Special Ordering Information
This is a radioactive product - shipping address must have a license to receive radioactive materials.
Storage Conditions
-80°C
Unit Size
250 µCi

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