AlphaLISA Human NGAL Detection Kit, 5,000 Assay Points

Formats:

• Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

NGAL/lipocalin-2 is a 25 kDa protein that is generated by inflammatory cells when encountering bacteria. Binding of bacteria to toll-like receptors generate the secretion of NGAL, which binds to bacterial iron-binding proteins and deactivates them. It is also secreted upon stimulation of immune cells by inflammatory cytokines such as TNF. NGAL shows elevated levels in many pathologies related to inflammation such as inflammation, pneumonia, kidney failure, obesity, cancer, meningitis, bladder infection, Alzeimer’s disease and others. As such, it is a valuable marker in all sort of pathologies in medias such as serum, plasma, urine and even saliva. It is also secreted in large amounts by some cancer cells.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.