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Application Note

Western blot validation of CRISPR-Cas9-induced functional knockout using synthetic sgRNA molecules

western-lightening-one-crispr512

Any well-designed CRISPR experiment must include a method to assess the accuracy and reliability of predicted gene editing results. This application note demonstrates how Revvity’s Edit-R from its Horizon Discovery product line predesigned synthetic sgRNAs result in high efficacy knockout of target genes as validated by western blot using Revvity’s Western Lightning ONE chemiluminescent substrates.

For research use only. Not for use in diagnostic procedures. 

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Western blot validation of CRISPR-Cas9-induced functional knockout using synthetic sgRNA molecules