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Application Note

Kinetic analysis of calcium flux activity in human iPSC-derived neurons using the Opera Phenix Plus system

calcium-flux512

The study of intracellular dynamic processes is of fundamental importance for unraveling the mechanisms of a broad spectrum of diseases and to develop better drugs and therapies. Particularly in the field of neuroscience and neurodegenerative diseases calcium signaling is of critical importance.

This application note demonstrates fast kinetic imaging using the Opera Phenix Plus HCS system to visualize and evaluate calcium flux activity in single human iPSC-derived neurons labeled with PhenoVue® Cal-520AM.

Key features:

  • Fast frame rate imaging to accurately capture rapid cellular responses
  • Reliable quantification of calcium activity in single neurons
  • Measure immediate compound responses using onboard liquid handling

For research use only. Not for use in diagnostic procedures. 

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Kinetic analysis of calcium flux activity in human iPSC-derived neurons using the Opera Phenix Plus system

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