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  • Immunoassays
  • HTRF
  • HTRF Anti-VHH d2-Antibody, 5,000 Assay Points

HTRF Anti-VHH d2-Antibody, 5,000 Assay Points

Generic HTRF MAb d2 image
HTRF Anti-VHH d2-Antibody, 5,000 Assay Points
Generic HTRF MAb d2 image
Generic HTRF MAb d2 image
Generic HTRF MAb d2 image

HTRF Anti-VHH-d2 can be used to capture alpaca and llama VHH nanobodies.

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Feature Specification
Application Protein-Protein Interaction
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HTRF Anti-VHH-d2 can be used to capture alpaca and llama VHH nanobodies.

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Product variants
Unit Size: 5,000 assay points
Part #:
610VHHDLA
List price
USD 677.30
Your online price:
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Unit Size: 20,000 assay points
Part #:
610VHHDLB
List price
USD 2,166.32
Your online price:
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For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
Total list price:
USD 677.30
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Generic HTRF MAb d2 image
HTRF Anti-VHH d2-Antibody, 5,000 Assay Points
Generic HTRF MAb d2 image
Generic HTRF MAb d2 image
HTRF Anti-VHH d2-Antibody, 5,000 Assay Points
Generic HTRF MAb d2 image
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Product information

  • Overview
  • How it works
  • Specifications

Overview

Monoclonal anti-VHH antibody has been labeled with d2 and binds to the native form of both alpaca and llama VHH, as well as recombinant nanobodies. The binding is rapid and stable, making it an ideal choice for use in a variety of assays including nanobody selection and titration.

How it works

Assay principle

In an HTRF interaction assay, one partner is labeled (directly or indirectly) with the donor, and the other with the acceptor (again, directly or indirectly). The intensity of the resulting signal is proportional to the binding of the 2 partners. In the example shown here, anti-VHH-d2 binds to the nanobodies raised against protein A, while partner A* binds to a specific Ab labeled with an HTRF donor.

*partner A can also be biotinylated, tagged, or Fc fused. In these cases, use the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin) labeled with a donor for the detection.

assay principle Anti-VHH d2
Assay protocol

The example on the right describes the protocol using a 20 µL final assay volume for the detection of an interaction between a nanobody raised against protein A and a non-tagged partner A*. Dispense the two partners (10 µL): i.e. protein A+nanobody, add anti-partner A labeled with donor (5 µL), and anti-VHH-d2 (5 µL) Then incubate, and finally read.

*partner A can also be biotinylated, tagged or Fc fused. In these cases, use the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin), labeled with a donor for the detection.

assay protocol Anti-VHH d2

 

Specifications

Application
Protein-Protein Interaction
Brand
HTRF
Detection Modality
HTRF
Product Group
Fluorescent Reagent
Shipping Conditions
Shipped Ambient
Target Class
Binding Assay
Technology
TR-FRET
Unit Size
5,000 assay points

Resources

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Infographic
A brief history on protein-protein interactions

Proteins and their interactions have a history of keen research and technological innovations. All scientists deciphering the...

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Infographic
HTRF protein-protein interaction: your dream assay served on a plate

Protein-protein interactions are like sandwiches: there’s no end in sight and no one knows what yours need better than you do.

With...

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