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NEXTFLEX Cell Free DNA-Seq Library Prep Kit 2.0

The NEXTFLEX®  Cell Free DNA-Seq Kit 2.0 can produce libraries from 10 ng of cell free DNA for circulating tumor DNA (ctDNA) or cell free fetal DNA (cffDNA) analysis, for sequencing on Illumina® or Element Biosciences® NGS platforms in two hours.

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MARCH10
Feature Specification
Automation Compatible Yes
Product Group DNA-seq

The NEXTFLEX®  Cell Free DNA-Seq Kit 2.0 can produce libraries from 10 ng of cell free DNA for circulating tumor DNA (ctDNA) or cell free fetal DNA (cffDNA) analysis, for sequencing on Illumina® or Element Biosciences® NGS platforms in two hours.

Click to copy promo code to clipboard.
March lab savings - SAVE 10%. Use promo code below.
MARCH10
Product Variants
Unit Size: 8 rxns
Part #:
NOVA-5150-01
List Price
USD 192.00
Your online price:
Unit Size: 48 rxns
Part #:
NOVA-5150-02
List Price
USD 1,152.00
Your online price:
For research use only. Not for use in diagnostic procedures.
Total list price:
USD 1,152.00
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Supporting products you might need

Overview

  • Designed for low sample input – 10 ng of input cfDNA required
  • Accelerated workflow with minimized hands-on time
  • Flexible adapter barcode options:
    • Up to 1,536 unique dual index barcodes are available.
    • Up to 96 UDI-UMI Barcodes now available to improve the detection of rare variants.
  • Compatible with Illumina® and Element Biosciences® sequencing platforms
  • Protocols available for automation on the Sciclone® G3 NGSx, NGSx iQ™ and Zephyr® G3 NGS workstations

The NEXTFLEX® Cell Free DNA-Seq Kit 2.0 delivers high coverage and reduced bias for Illumina® sequencing applications. The NEXTFLEX® Cell Free DNA-Seq Kit 2.0 can be used to prepare single, paired-end and multiplexed DNA libraries for sequencing using Illumina® platforms. The availability of up to 1,536 unique dual index barcodes facilitates high-throughput applications without compromising data integrity.

Reduced Bias and Coverage Gaps

NEXTFLEX® Cell Free DNA-Seq Kit 2.0 exhibits minimal GC bias and produces uniform coverage of difficult to sequence genome regions. This kit also offers robust ligation efficiency, resulting in greater library diversity and a larger number of unique sequencing reads. These optimizations enable investigators to obtain more information from each sequencing run.

Flexible Multiplexing Options

The NEXTFLEX® Cell Free DNA-Seq Library Prep Kit 2.0 is designed to be used with the NEXTFLEX® Unique Dual Index barcodes (384 barcodes) or the NEXTFLEX® Unique Dual Index barcodes (1,536 barcodes). NEXTFLEX UDI-UMI adapters, incorporating molecular barcodes into each adapter, are also available for applications involving the detection of rare variants.

Specifications

Automation Compatible
Yes
Product Group
DNA-seq
Shipping Conditions
Dual Temperature
Unit Size
48 rxns

SDS, COAs, Manuals and more

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Adapters (10NT index)

Revvity kit box for reagents and consumables
Part number:NOVA-534100
List price:USD 20,655.00/each
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USD 0
Revvity kit box for reagents and consumables
Part number:NOVA-534101
List price:USD 5,164.26/each
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USD 0
Revvity kit box for reagents and consumables
Part number:NOVA-534102
List price:USD 5,164.26/each
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USD 0
Revvity kit box for reagents and consumables
Part number:NOVA-534103
List price:USD 5,164.26/each
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USD 0
Revvity kit box for reagents and consumables
Part number:NOVA-534104
List price:USD 5,164.26/each
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USD 0

Adapters (8NT index)

Revvity kit box for reagents and consumables
Part number:NOVA-514150
List price:USD 1,958.40/each
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USD 0
Revvity kit box for reagents and consumables
Part number:NOVA-514150-EVAL16
List price:USD 163.20/each
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USD 0
Revvity kit box for reagents and consumables
Part number:NOVA-514150-EVAL48
List price:USD 489.60/each
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USD 0
Revvity kit box for reagents and consumables
Part number:NOVA-514151
List price:USD 1,958.40/each
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USD 0
Revvity kit box for reagents and consumables
Part number:NOVA-514152
List price:USD 1,958.40/each
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USD 0

Targeted Sequencing

Revvity kit box for reagents and consumables
Part number:NOVA-5143231
List price:USD 113.41/each
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USD 0
Revvity kit box for reagents and consumables
Part number:NOVA-5143232
List price:USD 237.54/each
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USD 0
Revvity kit box for reagents and consumables
Part number:NOVA-5143233
List price:USD 373.42/each
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USD 0

UDI-UMI Barcode Adapters

Revvity kit box for reagents and consumables
Part number:NOVA-734100
List price:USD 57.12/each
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USD 0
Revvity kit box for reagents and consumables
Part number:NOVA-734101
List price:USD 171.36/each
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USD 0
Revvity kit box for reagents and consumables
Part number:NOVA-734102
List price:USD 342.72/each
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Revvity kit box for reagents and consumables
Part number:NOVA-734103
List price:USD 685.44/each
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USD 0

cfDNA Extraction

chemagic Kits 700x700
Part number:CMG-1304
List price:USD 7,070.83/each
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USD 0
chemagic Kits 700x700
Part number:CMG-134
List price:USD 991.37/each
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