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AlphaLISA SureFire Ultra Human Total LCK Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human Total LCK Detection Kit, Control Lysate
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Total LCK assay. This control is already provided in the AlphaLISA SureFire Ultra Total LCK assay kit, but can also be ordered separately.
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Total LCK assay. This control is already provided in the AlphaLISA SureFire Ultra Total LCK assay kit, but can also be ordered separately.
AlphaLISA SureFire Ultra Human Total LCK Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human Total LCK Detection Kit, Control Lysate
Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Total LCK Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. The lysate is prepared from Jurkat cells, cultured in a T175 flask to approximately 1 x 106 cells/mL in 10% FBS containing medium. Cells were resuspended in HBSS at 4 x 106 cells/mL and treated with 11 mM Peroxide plus 50 nM Calyculin A for 10 minutes. Cells were lysed with 5X SureFire Ultra Lysis Buffer at a final density of 3.2 x 106 cells/mL.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Total
|
| Product Group |
Control Lysate
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
LCK
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Unit Size |
1 Vial
|
Video gallery
AlphaLISA SureFire Ultra Human Total LCK Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human Total LCK Detection Kit, Control Lysate
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