Cross-adsorbed donkey anti-rabbit IgG (H+L) antibody conjugated with PhenoVue™ Fluor 488.
PhenoVue Fluor 488 secondary antibody conjugates exhibit bright green fluorescence and are validated for use in high-content screening applications.
Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue Fluor - 488 donkey anti-rabbit antibody, cross-adsorbed, has a maximum excitation wavelength of 495nm and a maximum emission wavelength of 520nm.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
Feature | Specification |
---|---|
Color | Green |
Filter | FITC |
Fluorophore | PhenoVue™ Fluor 488 |
Host Species | Donkey |
Target Species | Rabbit |
Cross-adsorbed donkey anti-rabbit IgG (H+L) antibody conjugated with PhenoVue™ Fluor 488.
PhenoVue Fluor 488 secondary antibody conjugates exhibit bright green fluorescence and are validated for use in high-content screening applications.
Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue Fluor - 488 donkey anti-rabbit antibody, cross-adsorbed, has a maximum excitation wavelength of 495nm and a maximum emission wavelength of 520nm.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
PhenoVue Fluor 488 - donkey anti-rabbit IgG (H+L) antibody, cross-adsorbed, is affinity purified and recognizes rabbit IgG. It can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
PhenoVue Fluor 488 - donkey anti-rabbit IgG (H+L) antibody, cross-adsorbed, may display cross-reactivity with other species such as human or Guinea pig. For improved specificity and lower background, or in multiplexed applications, consider using highly cross-adsorbed PhenoVue Fluor conjugated secondary antibodies as an alternative.
Numbers of vials per unit | 1 |
---|---|
Quantity or volume per unit | 1 mg |
Form | Lyophilized |
Storage | 2-8°C |
Recommended working concentration | 1.5 µg/mL (10nM) |
Maximum excitation wavelength | 495 nm |
Maximum emission wavelength | 520 nm |
Common filter set | FITC |
Live cell staining | No |
Fixed cell staining | Yes |
Equivalent number of microplates | 25-70 x 96-well microplates 20-70 x 384-well microplates 35-90 x 1536-well microplates |
Color |
Green
|
---|---|
Form |
Lyophilized
|
Maximum Emission Wavelength (Emmax) |
520 nm
|
Maximum Excitation Wavelength (Exmax) |
495 nm
|
Application |
High Content Imaging
Microscopy
|
---|---|
Brand |
PhenoVue™
|
Cross-Adsorption |
Cross-adsorbed
|
Detection Modality |
Fluorescence
|
Filter |
FITC
|
Fluorophore |
PhenoVue™ Fluor 488
|
Host Species |
Donkey
|
Quantity |
1 x 1 mg
|
Sample Type |
Fixed samples only
|
Shipping Conditions |
Shipped Ambient
|
Storage Conditions |
2-8 °C, protected from light
|
Target Species |
Rabbit
|
Type |
Individual Reagent
|
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