LANCE Ultra phospho-ZAP-70 (Tyr319) kits are designed for the detection of phosphorylated ZAP-70 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Feature | Specification |
---|---|
Application | Cell Signaling |
Sample Volume | 15 µL |
LANCE Ultra phospho-ZAP-70 (Tyr319) kits are designed for the detection of phosphorylated ZAP-70 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Please note control lysates are sold separately, catalog number TRF4024S.
Formats:
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
ZAP-70 (Zeta-chain-associated protein kinase 70) is normally expressed near the surface membrane of T-cells and natural killer cells, but levels in B-cells can be an indicator for various forms of leukemia. ZAP-70 is recruited to the TCR/CD3 complex in order to be phosphorylated. Once phosphorylated, ZAP-70 promotes downstream phosphorylation of proteins such as SLP76 and ERK.
Application |
Cell Signaling
|
---|---|
Automation Compatible |
Yes
|
Brand |
LANCE Ultra
|
Detection Modality |
LANCE
|
Molecular Modification |
Phosphorylation
|
Product Group |
Kit
|
Sample Volume |
15 µL
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
Zap70
|
Target Class |
Phosphoproteins
|
Technology |
TR-FRET
|
Therapeutic Area |
Autoimmunity
Immuno-oncology
Oncology
|
Unit Size |
500 Assay Points
|
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LANCE™ Ultra TR-FRET is a no-wash proximity assay technology that combines the benefits of time resolution (TR) with fluorescence...
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