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Alpha Citations

On this page we provide selected citations for each application.

AlphaLISAâ„¢ immunoassays for biomarker detection

Journal articles
  1. Huertas A, Tu L, Gambaryan N, Girerd B, Perros F, Montani D, Fabre D, Fadel E, Eddahibi S, Cohen-Kaminsky S, Guignabert C, Humbert M. Leptin and regulatory T lymphocytes in idiopathic pulmonary arterial hypertension. Eur Respir J 2012 Feb. Link
  2. Freund A, Patil CK, Campisi J. p38MAPK is a novel DNA damage response-independent regulator of the senescence-associated secretory phenotype. EMBO J. 2011 Apr;30(8):1536–1548. Link
  3. Cauchon, E. et al. Development of a Homogeneous Immunoassay for the Detection of Angiotensin I in Plasma Using AlphaLISA Acceptor Beads Technology. Anal Biochem 388, 134-139 (2009) Link
  4. Lai, M.T. et al. Antiviral activity of MK-4965, A Novel Nonnucleoside Reverse Transcriptase Inhibitor. Antimicrob Agents Chemother 53, 2424-2431 (2009). Link
  5. Majercak, J. et al. LRRTM3 Promotes Processing of Amyloid-Precursor Protein by BACE1 and is a Positional Candidate Gene for Late-Onset Alzheimer's Disease. Proc Natl Acad Sci USA 103, 17967-17972 (2006). Link
  6. Petersen, S.B. et al. Comparison of a luminescent oxygen channeling immunoassay and an ELISA for detecting insulin aspart in human serum. J Pharm Biomed Anal 51, 217-224 (2010). Link
  7. Poulsen, F. and Jensen, K.B. A Luminescent Oxygen Channeling Immunoassay for the Determination of Insulin in Human Plasma. J Biomol Screen 12, 240-247 (2007). Link
  8. Szekeres, P.G. et al. Development of Homogeneous 384-well High-Throughput Screening Assays for Abeta1-40 and Abeta1-42 using AlphaScreen technology. J Biomol Screen 13, 101-111 (2008)

Biotherapeutics

  1. Houde D, Berkowitz SA. Conformational comparability of factor IX-Fc fusion protein, factor IX, and purified Fc fragment in the absence and presence of calcium. Journal of Pharmaceutical Sciences 2012 Jan. Link
  2. Goetze AM, Liu YD, Arroll T, Chu L, Flynn GC. Rates and Impact of Human Antibody Glycation In Vivo. Glycobiology 2011 Sep. Link
  3. Nakamura S, Morimoto N, Tsuruma K, Izuta H, Yasuda Y, Kato N, Ikeda T, Shimazawa M, Hara H. Tissue kallikrein inhibits retinal neovascularization via the cleavage of vascular endothelial growth factor-165. Arterioscler. Thromb. Vasc. Biol. 2011 May;31(5):1041–1048. Link
  4. Mazella J, Pétrault O, Lucas G, Deval E, Béraud-Dufour S, Gandin C, El-Yacoubi M, Widmann C, Guyon A, Chevet E, Taouji S, Conductier G, Corinus A, Coppola T, Gobbi G, Nahon J-L, Heurteaux C, Borsotto M. Spadin, a sortilin-derived peptide, targeting rodent TREK-1 channels: a new concept in the antidepressant drug design. PLoS Biol 2010;8(4):e1000355. Link
  5. Saitoh R, Ohtomo T, Yamada Y, Kamada N, Nezu J-I, Kimura N, Funahashi S-I, Furugaki K, Yoshino T, Kawase Y, Kato A, Ueda O, Jishage K-I, Suzuki M, Fukuda R, Arai M, Iwanari H, Takahashi K, Sakihama T, Ohizumi I, Kodama T, Tsuchiya M, Hamakubo T. Viral envelope protein gp64 transgenic mouse facilitates the generation of monoclonal antibodies against exogenous membrane proteins displayed on baculovirus. J. Immunol. Methods 2007 Apr;322(1-2):104–117. Link
  6. Lazar GA, Dang W, Karki S, Vafa O, Peng JS, Hyun L, Chan C, Chung HS, Eivazi A, Yoder SC, Vielmetter J, Carmichael DF, Hayes RJ, Dahiyat BI. Engineered antibody Fc variants with enhanced effector function. Proc. Natl. Acad. Sci. U.S.A 2006 Mar;103(11):4005–10. Link
  7. Miller MD, Geleziunas R, Bianchi E, Lennard S, Hrin R, Zhang H, Lu M, An Z, Ingallinella P, Finotto M, Mattu M, Finnefrock AC, Bramhill D, Cook J, Eckert DM, Hampton R, Patel M, Jarantow S, Joyce J, Ciliberto G, Cortese R, Lu P, Strohl W, Schleif W, McElhaugh M, Lane S, Lloyd C, Lowe D, Osbourn J, Vaughan T, Emini E, Barbato G, Kim PS, Hazuda DJ, Shiver JW, Pessi A. A human monoclonal antibody neutralizes diverse HIV-1 isolates by binding a critical gp41 epitope. Proc. Natl. Acad. Sci. U.S.A 2005 Oct;102(41):14759–64. Link

Protein-protein interactions

Journal articles
Cell-based protein-protein interactions
  1. Bovijn C, Ulrichts P, De Smet A-S, Catteeuw D, Beyaert R, Tavernier J, Peelman F. Identification of Interaction Sites for Dimerization and Adapter Recruitment in Toll/Interleukin-1 Receptor (TIR) Domain of Toll-like Receptor 4. J. Biol. Chem. 2012 Feb;287(6):4088–4098. Link
  2. Tang X, Seyb KI, Huang M, Schuman ER, Shi P, Zhu H, Glicksman MA. A High-Throughput Screening Method for Small-Molecule Inhibitors of the Aberrant Mutant SOD1 and Dynein Complex Interaction. J Biomol Screen 2012 Mar;17(3):314–326. Link
  3. Vosjan MJWD, Vercammen J, Kolkman JA, Stigter-van Walsum M, Revets H, van Dongen GAMS. Nanobodies targeting the hepatocyte growth factor: potential new drugs for molecular cancer therapy. Mol. Cancer Ther 2012 Feb. Link
  4. Becker MN, Todd TM, Moyer RW. An Amsacta moorei entomopoxvirus ortholog of the poly(A) polymerase small subunit exhibits methyltransferase activity and is non-essential for virus growth. Virology 2008 Jun;375(2):624–636. Link
  5. Mohamed MR, Rahman MM, Lanchbury JS, Shattuck D, Neff C, Dufford M, van Buuren N, Fagan K, Barry M, Smith S, Damon I, McFadden G. Proteomic screening of variola virus reveals a unique NF-kappaB inhibitor that is highly conserved among pathogenic orthopoxviruses. Proc. Natl. Acad. Sci. U.S.A 2009 Jun;106(22):9045–9050. Link
  6. Rahman MM, Mohamed MR, Kim M, Smallwood S, McFadden G. Co-regulation of NF-kappaB and inflammasome-mediated inflammatory responses by myxoma virus pyrin domain-containing protein m013. PLoS Pathog 2009 Oct;5(10):e1000635. Link
  7. Lavens D, Peelman F, Van der Heyden J, Uyttendaele I, Catteeuw D, Verhee A, Van Schoubroeck B, Kurth J, Hallenberger S, Clayton R, Tavernier J. Definition of the interacting interfaces of Apobec3G and HIV-1 Vif using MAPPIT mutagenesis analysis. Nucleic Acids Res 2009 Dec. Link
  8. Werden SJ, McFadden G. Pharmacological manipulation of the Akt signaling pathway regulates myxoma virus replication and tropism in human cancer cells. J Virol 2010 Jan;84(7):3287–3302. Link
  9. Waller H, Chatterji U, Gallay P, Parkinson T, Targett-Adams P. The use of AlphaLISA technology to detect interaction between hepatitis C virus-encoded NS5A and cyclophilin A. J Virol Methods 2010 Feb. Link

Biochemical protein-protein interactions

  1. Cavalluzzo C, Voet A, Christ F, Singh BK, Sharma A, Debyser Z, Maeyer MD, Eycken EV der. De novo design of small molecule inhibitors targeting the LEDGF/p75-HIV integrase interaction. RSC Advances 2012;2(3):974.
  2. Demeulemeester J, Tintori C, Botta M, Debyser Z, Christ F. Development of an AlphaScreen-Based HIV-1 Integrase Dimerization Assay for Discovery of Novel Allosteric Inhibitors. J Biomol Screen 2012 Feb. Link
  3. Houde D, Berkowitz SA. Conformational comparability of factor IX-Fc fusion protein, factor IX, and purified Fc fragment in the absence and presence of calcium. Journal of Pharmaceutical Sciences 2012 Jan. Link
  4. Madono M, Sawasaki T, Morishita R, Endo Y. Wheat germ cell-free protein production system for post-genomic research. N Biotechnol 2011 Apr;28(3):211–217. Link
  5. Goetze AM, Liu YD, Arroll T, Chu L, Flynn GC. Rates and Impact of Human Antibody Glycation In Vivo. Glycobiology 2011 Sep. Link
  6. Garcia BL, Summers BJ, Lin Z, Ramyar KX, Ricklin D, Kamath DV, Fu Z-Q, Lambris JD, Geisbrecht BV. Diversity in the C3B contact residues and tertiary structures of the staphylococcal complement inhibitor (SCIN) protein family. J Biol Chem 2011 Nov. Link
  7. Cassel JA, Blass BE, Reitz AB, Pawlyk AC. Development of a novel nonradiometric assay for nucleic acid binding to TDP-43 suitable for high-throughput screening using AlphaScreen technology. J Biomol Screen 2010 Oct;15(9):1099–1106. Link
  8. Kota S, Coito C, Mousseau G, Lavergne J-P, Strosberg AD. Peptide inhibitors of hepatitis C virus core oligomerization and virus production. J. Gen. Virol 2009 Jun;90(Pt 6):1319–1328. Link
  9. Lazar GA, Dang W, Karki S, Vafa O, Peng JS, Hyun L, Chan C, Chung HS, Eivazi A, Yoder SC, Vielmetter J, Carmichael DF, Hayes RJ, Dahiyat BI. Engineered antibody Fc variants with enhanced effector function. Proc. Natl. Acad. Sci. U.S.A 2006 Mar;103(11):4005–10. Link

DNA:protein or RNA:protein interactions

Journal articles
  1. McNeely M, Hendrix J, Busschots K, Boons E, Deleersnijder A, Gerard M, Christ F, Debyser Z. In vitro DNA tethering of HIV-1 integrase by the transcriptional coactivator LEDGF/p75. J. Mol. Biol 2011 Jul;410(5):811–830. Link
  2. Stutz A, Bertheloot D, Latz E. Innate immune receptors for nucleic acids. Methods Mol. Biol 2011;748:69–82. Link
  3. Dausse E, Taouji S, Evadé L, Di Primo C, Chevet E, Toulmé J-J. HAPIscreen, a method for high-throughput aptamer identification. J Nanobiotechnology 2011;9:25. Link
  4. Mills NL, Shelat AA, Guy RK. Assay Optimization and Screening of RNA-Protein Interactions by AlphaScreen. J Biomol Screen 2007 Oct;12(7):946–55. Link

Lipid:protein Interactions

Journal articles
  1. Malapaka RRV, Khoo SK, Zhang J, Choi JH, Zhou XE, Xu Y, Gong Y, Li J, Yong E-LE, Chalmers MJ, Chang L, Resau JH, Griffin PR, Chen YE, Xu HE. Identification and mechanism of a ten carbon fatty acid as a modulating ligand of peroxisome proliferator activated receptors. J Biol Chem 2011 Oct. Link
  2. Jerga A, Miller DJ, White SW, Rock CO. Molecular determinants for interfacial binding and conformational change in a soluble diacylglycerol kinase. J. Biol. Chem 2009 Mar;284(11):7246–7254. Link
  3. Rzepecki PW, Prestwich GD. Synthesis of hybrid lipid probes: derivatives of phosphatidylethanolamine-extended phosphatidylinositol 4,5-bisphosphate (Pea-PIP(2)). J. Org. Chem 2002 Aug;67(16):5454–5460. Link

Nuclear receptor assays

Journal articles
  1. Malapaka RRV, Khoo SK, Zhang J, Choi JH, Zhou XE, Xu Y, Gong Y, Li J, Yong E-LE, Chalmers MJ, Chang L, Resau JH, Griffin PR, Chen YE, Xu HE. Identification and mechanism of a ten carbon fatty acid as a modulating ligand of peroxisome proliferator activated receptors. J Biol Chem 2011 Oct. Link
  2. Istrate MA, Spicer TP, Wang Y, Bernard JA, Helvering LM, Bocchinfuso WP, Richardson TI, Zink R, Kumar N, Montrose-Rafizadeh C, Dodge J, Hodder P, Griffin PR. Development of an HTS-Compatible Assay for Discovery of ROR{alpha} Modulators Using AlphaScreen(R) Technology. J Biomol Screen 2011 Feb;16(2):183–191. Link
  3. Zhou XE, Suino-Powell KM, Xu Y, Chan C-W, Tanabe O, Kruse SW, Reynolds R, Engel JD, Xu HE. The Orphan Nuclear Receptor TR4 Is a Vitamin A-activated Nuclear Receptor. J. Biol. Chem 2011 Jan;286(4):2877–2885. Link
  4. Partch, C.L. & Card, P.B., Amezcua, C.A. & Gardner, K.H. Molecular Basis of Coiled Coil Coactivator Recruitment by the Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT). J Biol Chem 284, 15184-15192 (2009). Link
  5. Wagstaff, K.M. & Jans, D.A. Intramolecular Masking of Nuclear Localization Signals: Analysis of Importin Binding Using a novel AlphaScreen-based method. Anal Biochem 348, 49-56 (2006). Link
  6. Wang, W. et al. The Crystal Structures of Human Steroidogenic Factor-1 and Liver Receptor Homologue-1. Proc Natl Acad Sci USA 102, 7505-7510 (2005). Link
  7. Bramlett, K.S. et al. A Natural Product Ligand of the Oxysterol Receptor, Liver X Receptor. J Pharmacol Exp Ther 307, 291-296 (2003). Link
  8. Rouleau, N. et al. Development of a Versatile Platform for Nuclear Receptor Screening Using AlphaScreen. J Biomol Screen 8, 191-197 (2003). Link
  9. Xu H.E. et al. Structural Basis for Antagonist-Mediated Recruitment of Nuclear Co-Repressors by PPARalpha. Nature 415, 813-817 (2002). Link
  10. Glickman, J.F. et al. A Comparison of ALPHAScreen, TR-FRET, and TRF as Assay Methods for FXR Nuclear Receptors. J Biomol Screen 7, 3-10 (2002). Link

GPCRs

Journal articles
  1. Bartho JD, Ly K, Hay DL. Measurement of cAMP in an Undergraduate Teaching Laboratory, Using ALPHAscreen Technology. Sci. Signal. 2012 Feb;5(211):tr1. Link
  2. van Koppen CJ, de Gooyer ME, Karstens W-J, Plate R, Conti PGM, van Achterberg TAE, van Amstel MGA, Brands JHGM, Wat J, Berg RJW, Lane JRD, Miltenburg AMM, Timmers CM. Mechanism of Action of a Nanomolar Potent, Allosteric Antagonist of the Thyroid-Stimulating Hormone Receptor. Br. J. Pharmacol. 2011 Oct. Link
  3. Uitdehaag JCM, Sünnen CM, van Doornmalen AM, de Rouw N, Oubrie A, Azevedo R, Ziebell M, Nickbarg E, Karstens W-J, Ruygrok S. Multidimensional Profiling of CSF1R Screening Hits and Inhibitors: Assessing Cellular Activity, Target Residence Time, and Selectivity in a Higher Throughput Way. J Biomol Screen 2011 Aug. Link
  4. Hay DL. Measurement of Phosphorylated Extracellular Signal-Regulated Kinase 1 and 2 in an Undergraduate Teaching Laboratory with ALPHAscreen Technology. Sci. Signal. 2009 Mar;2(62):tr1. Link
  5. Pioszak, A.A., Parker, N.R., Suino-Powell, K. & Xu, H.E. Molecular Recognition of Corticotropin-Releasing Factor by its G-Protein-Coupled Receptor CRFR1. J Biol Chem 283, 32900-32912 (2008). Link
  6. Pioszak, A.A. & Xu, H.E. Molecular Recognition of Parathyroid Hormone by Its G Protein-Coupled Receptor. Proc Natl Acad Sci USA 105, 5034-5039 (2008). Link
  7. Siehler, S. Cell-Based Assays in GPCR Drug Discovery. Biotechnol J 3, 471-483 (2008). Link

Kinases

Journal articles
Biochemical
  1. Arai R, Tsuda M, Watanabe T, Ose T, Obuse C, Maenaka K, Minami A, Ohba Y. Simultaneous inhibition of Src and Aurora kinases by SU6656 induces therapeutic synergy in human synovial sarcoma growth, invasion and angiogenesis in vivo. European Journal of Cancer (Oxford, England: 1990) 2012 Jan. Link
  2. Gao Z-G, Verzijl D, Zweemer A, Ye K, Göblyös A, Ijzerman AP, Jacobson KA. Functionally biased modulation of A(3) adenosine receptor agonist efficacy and potency by imidazoquinolinamine allosteric enhancers. Biochem. Pharmacol. 2011 Sep;82(6):658–668. Link
  3. Babij C, Zhang Y, Kurzeja RJ, Munzli A, Shehabeldin A, Fernando M, Quon K, Kassner PD, Ruefli-Brasse AA, Watson VJ, Fajardo F, Jackson A, Zondlo J, Sun Y, Ellison AR, Plewa CA, San MT, Robinson J, McCarter J, Schwandner R, Judd T, Carnahan J, Dussault I. STK33 Kinase Activity Is Nonessential in KRAS-Dependent Cancer Cells. Cancer Res 2011 Sep;71(17):5818–5826. Link
  4. Peterson EA, Andrews PS, Be X, Boezio AA, Bush TL, Cheng AC, Coats JR, Colletti AE, Copeland KW, DuPont M, Graceffa R, Grubinska B, Harmange J-C, Kim JL, Mullady EL, Olivieri P, Schenkel LB, Stanton MK, Teffera Y, Whittington DA, Cai T, La DS. Discovery of triazine-benzimidazoles as selective inhibitors of mTOR. Bioorg. Med. Chem. Lett 2011 Apr;21(7):2064–2070. Link
  5. Li, Y. et al. Homogeneous Assays for Adenosine 5'-Monophosphate-Activated Protein Kinase. Anal Biochem 321, 151-156 (2003). Link
  6. Von Leoprechting, A. et al. Miniaturization and Validation of a High-Throughput Serine Kinase Assay Using the AlphaScreen Platform. J Biomol Screen 9, 719-725 (2004). Link
  7. Warner, G. et al. AlphaScreen Kinase HTS Platforms. Curr Med Chem 11, 721-730 (2004). Link

Cellular

  1. Allen JV, Bardelle C, Blades K, Buttar D, Chapman L, Colclough N, Dossetter AG, Garner AP, Girdwood A, Lambert C, Leach AG, Law B, Major J, Plant H, Slater AM. The discovery of benzanilides as c-Met receptor tyrosine kinase inhibitors by a directed screening approach. Bioorg. Med. Chem. Lett 2011 Sep;21(18):5224–5229. Link
  2. Babij C, Zhang Y, Kurzeja RJ, Munzli A, Shehabeldin A, Fernando M, Quon K, Kassner PD, Ruefli-Brasse AA, Watson VJ, Fajardo F, Jackson A, Zondlo J, Sun Y, Ellison AR, Plewa CA, San MT, Robinson J, McCarter J, Schwandner R, Judd T, Carnahan J, Dussault I. STK33 kinase activity is nonessential in KRAS-dependent cancer cells. Cancer Res 2011 Sep;71(17):5818–5826. Link
  3. Drew AE, Al-Assaad S, Yu V, Andrews P, Merkel P, Szilvassy S, Emkey R, Lewis R, Brake RL. Comparison of 2 Cell-Based Phosphoprotein Assays to Support Screening and Development of an ALK Inhibitor. J Biomol Screen 2011 Feb;16(2):164–173. Link
  4. Boldyreff, B. et al. Expression and Purification of PI3 Kinase Alpha and Development of an ATP Depletion and an Alphascreen PI3 Kinase Activity Assay. J Biomol Screen 13, 1035-1040 (2008). Link
  5. Guenat, S. et al. Homogeneous and Nonradioactive High-Throughput Screening Platform for the Characterization of Kinase Inhibitors in Cell Lysates. J Biomol Screen 11, 1015-1026 (2006). Link
  6. Hay, D.L. Measurement of Phosphorylated Extracellular Signal-Regulated Kinase 1 and 2 in an Undergraduate Teaching Laboratory with ALPHAscreen Technology Sci Signal 2, tr1 (2009). Link
  7. Smotrov, N. et al. Development of a Cell-Based Assay for Measurement of c-Met Phosphorylation Using AlphaScreen Technology and High-Content Imaging Analysis. J Biomol Screen 14, 404-411 (2009). Link

Epigenetics and post-translational modifications

Journal articles
  1. Herold, J. M. et al. Structure–activity relationships of methyl-lysine reader antagonists. MedChemComm3, 45 (2012).
  2. Luo M. Current Chemical Biology Approaches to Interrogate Protein Methyltransferases. ACS Chemical Biology 2012 Jan. Link
  3. Falk H, Connor T, Yang H, Loft KJ, Alcindor JL, Nikolakopoulos G, Surjadi RN, Bentley JD, Hattarki MK, Dolezal O, Murphy JM, Monahan BJ, Peat TS, Thomas T, Baell JB, Parisot JP, Street IP. An Efficient High-Throughput Screening Method for MYST Family Acetyltransferases, a New Class of Epigenetic Drug Targets. J Biomol Screen 2011 Nov. Link
  4. Guo W, Qian L, Zhang J, Zhang W, Morrison A, Hayes P, Wilson S, Chen T, Zhao J. Sirt1 overexpression in neurons promotes neurite outgrowth and cell survival through inhibition of the mTOR signaling. J. Neurosci. Res 2011 Nov;89(11):1723–1736. Link
  5. Philpott M, Yang J, Tumber T, Fedorov O, Uttarkar S, Filippakopoulos P, Picaud S, Keates T, Felletar I, Ciulli A, Knapp S, Heightman TD. Bromodomain-peptide displacement assays for interactome mapping and inhibitor discovery. Mol Biosyst 2011 Oct;7(10):2899–2908. Link
  6. Hauser A-T, Bissinger E-M, Metzger E, Repenning A, Bauer U-M, Mai A, Schüle R, Jung M. Screening Assays for Epigenetic Targets Using Native Histones as Substrates. J Biomol Screen 2011 Sep. Link
  7. Gauthier N, Caron M, Pedro L, Arcand M, Blouin J, Labonté A, Normand C, Paquet V, Rodenbrock A, Roy M, Rouleau N, Beaudet L, Padrós J, Rodriguez-Suarez R. Development of Homogeneous Nonradioactive Methyltransferase and Demethylase Assays Targeting Histone H3 Lysine 4. J Biomol Screen 2011 Sep. Link
  8. Goetze AM, Liu YD, Arroll T, Chu L, Flynn GC. Rates and Impact of Human Antibody Glycation In Vivo. Glycobiology 2011 Sep. Link
  9. Ferguson AD, Larsen NA, Howard T, Pollard H, Green I, Grande C, Cheung T, Garcia-Arenas R, Cowen S, Wu J, Godin R, Chen H, Keen N. Structural Basis of Substrate Methylation and Inhibition of SMYD2. Structure 2011 Sep;19(9):1262–1273. Link
  10. Quinn, A. et al. A homogeneous method for investigation of methylation-dependent protein-protein interactions in epigenetics. Nucl Acids Res , 1-10 (2009). Link
  11. Salahudeen, A.A. et al. An E3 ligase possessing an iron-responsive hemerythrin domain is a regulator of iron homeostasis. Science 326, 722-726 (2009). Link
  12. Wigle, T.J. et al. Screening for Inhibitors of Low-Affinity Epigenetic Peptide-Protein Interactions: An AlphaScreen-Based Assay for Antagonists of Methyl-Lysine Binding Proteins. J Biomol Screen (2009). Link
  13. Wallace, M. et al. Dual-Site Regulation of MDM2 E3-Ubiquitin Ligase Activity. Mol Cell 23, 251-263 (2006). Link
  14. Kus, B. et al. A High Throughput Screen to Identify Substrates for the Ubiquitin Ligase Rsp5. J Biol Chem 280, 29470-29478 (2005). Link

Proteases and other enzymatic assays

Journal articles
  1. Akagi T, Shimizu K, Takahama S, Iwasaki T, Sakamaki K, Endo Y, Sawasaki T. Caspase-8 cleavage of the interleukin-21 (IL-21) receptor is a negative feedback regulator of IL-21 signaling. FEBS Lett 2011 Jun;585(12):1835–1840. Link
  2. Madono M, Sawasaki T, Morishita R, Endo Y. Wheat germ cell-free protein production system for post-genomic research. N Biotechnol 2011 Apr;28(3):211–217. Link
  3. Marchand, C. et al. Identification of Phosphotyrosine Mimetic Inhibitors of Human Tyrosyl-DNA Phosphodiesterase I by a Novel AlphaScreen High-Throughput Assay. Mol Cancer Ther 8, 240-248 (2009). Link
  4. Espeseth, A.S. et al. Compounds that Bind APP and Inhibit Abeta Processing in Vitro Suggest a Novel Approach to Alzheimer Disease Therapeutics. J Biol Chem 280, 17792-17797 (2005). Link
  5. Peppard. J. et al. Development of a High-Throughput Screening Assay for Inhibitors of Aggrecan Cleavage Using Luminescent Oxygen Channeling (AlphaScreen). J Biomol Screen 8, 149-156 (2003). Link

Antibody screening and detection

Journal articles
  1. Vosjan MJWD, Vercammen J, Kolkman JA, Stigter-van Walsum M, Revets H, van Dongen GAMS. Nanobodies targeting the hepatocyte growth factor: potential new drugs for molecular cancer therapy. Mol. Cancer Ther 2012 Feb. Link
  2. Goetze AM, Liu YD, Arroll T, Chu L, Flynn GC. Rates and Impact of Human Antibody Glycation In Vivo. Glycobiology 2011 Sep. Link
  3. Stewart R, Thom G, Levens M, Güler-Gane G, Holgate R, Rudd PM, Webster C, Jermutus L, Lund J. A variant human IgG1-Fc mediates improved ADCC. Protein Eng. Des. Sel. 2011 Sep;24(9):671–678. Link
  4. Tsuge S, Mizutani Y, Matsuoka K, Sawasaki T, Endo Y, Naruishi K, Maeda H, Takashiba S, Shiogama K, Inada K-I, Tsutsumi Y. Specific in situ visualization of plasma cells producing antibodies against Porphyromonas gingivalis in gingival radicular cyst: application of the enzyme-labeled antigen method. J. Histochem. Cytochem 2011 Jul;59(7):673–689. Link
  5. Bembenek ME, Burkhardt A, Ma J, Li Z, Loke H-K, Wu D, Xu Q, Tayber O, Xie L, Li P, Li L. Determination of complementary antibody pairs using protein A capture with the AlphaScreen assay format. Anal. Biochem 2011 Jan;408(2):321–327. Link
  6. Lazar, G.A. et al. Engineered Antibody Fc Variants With Enhanced Effector Function. Proc Natl Acad Sci USA 103, 4005-4010 (2006). Link
  7. Miller, M.D. et al. A Human Monoclonal Antibody Neutralizes Diverse HIV-1 Isolates by Binding a Critical gp41 Epitope. Proc Natl Acad Sci USA 102, 14759-14764 (2005). Link

cAMP assays

  1. Bartho JD, Ly K, Hay DL. Measurement of cAMP in an Undergraduate Teaching Laboratory, Using ALPHAscreen Technology. Sci. Signal. 2012 Feb;5(211):tr1. Link
  2. Bailey RJ, Walker CS, Ferner AH, Loomes KM, Prijic G, Halim A, Whiting L, Phillips ARJ, Hay DL. Pharmacological characterisation of rat amylin receptors: implications for the identification of amylin receptor subtypes. Br. J. Pharmacol. 2011 Oct. Link
  3. Qiu Y, Wang Y, Law P-Y, Chen H-Z, Loh HH. Cholesterol regulates micro-opioid receptor-induced beta-arrestin 2 translocation to membrane lipid rafts. Mol. Pharmacol 2011 Jul;80(1):210–218. Link
  4. Breit, A., Lagacé, M. & Bouvier, M. Hetero-Oligomerization Between Beta2- and Beta3-Adrenergic Receptors Generates a Beta-Adrenergic Signaling Unit With Distinct Functional Properties. J Biol Chem 279, 28756-28765 (2004). Link
  5. Elster, L., Elling, C. & Heding, A. Bioluminescence Resonance Energy Transfer as a Screening Assay: Focus on Partial and Inverse Agonism. J Biomol Screen 12, 41-49 (2007). Link
  6. Moll, D. et al. Biomolecular Interaction Analysis in Functional Proteomics. J Neural Transm 113, 1015-1032 (2006). Link
  7. Slominski, A., et al. CRH Functions as a Growth Factor/Cytokine in the Skin. J Cell Physiol 206, 780-791 (2006). Link

cGMP assays

Journal articles
  1. St Pierre, L. et al. Cloning and Characterisation of Natriuretic Peptides from the Venom Glands of Australian Elapids. Biochimie 88, 1923-1931 (2006). Link
  2. Tessler, R. et al. Sildenafil Acutely Reverses the Hypoxic Pulmonary Vasoconstriction Response of the Newborn Pig. Pediatric Research 64, 251-255 (2008). Link

Working with cell extracts and supernatants

Journal articles
  1. Bembenek ME, Burkhardt A, Ma J, Li Z, Loke H-K, Wu D, Xu Q, Tayber O, Xie L, Li P, Li L. Determination of complementary antibody pairs using protein A capture with the AlphaScreen assay format. Anal. Biochem 2011 Jan;408(2):321–327. Link
  2. Tang X, Seyb KI, Huang M, Schuman ER, Shi P, Zhu H, Glicksman MA. A High-Throughput Screening Method for Small-Molecule Inhibitors of the Aberrant Mutant SOD1 and Dynein Complex Interaction. J Biomol Screen 2012 Mar;17(3):314–326. Link
  3. Bammens L, Chavez-Gutierrez L, Tolia A, Zwijsen A, De Strooper B. Functional and topological analysis of Pen-2, the fourth subunit of the gamma-secretase complex. J Biol Chem 2011 Feb. Link
  4. Waller H, Chatterji U, Gallay P, Parkinson T, Targett-Adams P. The use of AlphaLISA technology to detect interaction between hepatitis C virus-encoded NS5A and cyclophilin A. J Virol Methods 2010 Feb. Link
  5. Szekeres, P.G. et al. Development of Homogeneous 384-Well High-Throughput Screening Assays for Abeta1-40 and Abeta1-42 Using AlphaScreen Technology. J Biomol Screen 2008;13(2):101-11. Link
  6. Guenat, S. et al. Homogeneous and Nonradioactive High-Throughput Screening Platform for the Characterization of Kinase Inhibitors in Cell Lysates. J Biomol Screen 11, 1015-1026 (2006). Link

SNP detection assays

Journal articles
  1. Beaudet, L. et al. Homogeneous Assays for Single-Nucleotide Polymorphism Typing Using AlphaScreen. Genome Res 11, 600-608 (2001). Link

GTPase assays

Journal articles
  1. Niebel, B. et al. A luminescent oxygen channeling biosensor that measures small GTPase activation. Chem. Commun. (Camb.)47, 7521–7523 (2011). Link
  2. Caruso, M. et al. Biochemical Clustering of Monomeric GTPases of the Ras Superfamily. Mol Cell Proteomics 4, 936-944 (2005). Link

Phosphodiesterase assays

Journal articles
  1. Weidlich IE, Dexheimer T, Marchand C, Antony S, Pommier Y, Nicklaus MC. Inhibitors of human tyrosyl-DNA phospodiesterase (hTdp1) developed by virtual screening using ligand-based pharmacophores. Bioorg. Med. Chem 2009 Nov. Link
  2. Marchand, C. et al. Identification of Phosphotyrosine Mimetic Inhibitors of Human Tyrosyl-DNA Phosphodiesterase I by a Novel AlphaScreen High-Throughput Assay. Mol Cancer Ther 8, 240-248 (2009). Link

 

For research use only. Not for use in diagnostic procedures.