Skip to main content
Menu
US

ViaStain Cell Fitness Panel for Cellometer Spectrum

The Cell Fitness Panel is comprised of 10 components to measure the 5 assays on Cellometer Spectrum.

Feature Specification
Assay Points 125 tests
Instrument Compatibility Cellometer Spectrum Image Cytometry System

The Cell Fitness Panel is comprised of 10 components to measure the 5 assays on Cellometer Spectrum.

Product Variant
Quantity: 1 kit
Part #:
CSK-V0023-1
List Price
USD 910.90
For research use only. Not for use in diagnostic procedures.

Overview

For use on Cellometer Spectrum.

  • Total of 125 tests. Viability - AO/PI (Acridine Orange and Propidium Iodide) - Using AO/PI allows for the identification, enumeration of mononuclear cells, and provides cell viability in the counted sample without a RBC lysing steps
  • Vitality/Enzymatic Activity - Calcein AM/PI - By looking at the conversion of non-fluorescent calcein AM to green fluorescent calcein we can measure the number and concentration of both metabolically active live cells as well as non-viable cells in a given population.
  • Reactive Oxygen Species - Total ROS - While reactive oxygen species (ROS) are natural by-products of normal metabolism, during oxidative stress-related events, the accumulation of ROS can result in significant damage to cell structures. Cultures that are positive for ROS may signal unhealthy culture conditions that can lead to apoptosis and cell death.
  • Mid-Stage Apoptosis - Annexin V/PI - An assay commonly carried out by researchers interested in performing functional assays to measure the percent of live, apoptotic, and necrotic cells in a sample. May serve as an early indicator of an unhealthy cell population.
  • Late-Stage Apoptosis - Caspase 3/7 - Early or mid-stage apoptotic cells may recover or may progress to late stage apoptosis. A cell culture with a high number of caspase 3/7 positive cells may be in trouble. In this assay the executioner caspase is detected as a bright nuclear signal.

Instrument Compatibility: The Cell Fitness Panel is comprised of 10 components to measure the 5 assays described below. The parameters include a measurement of: viability, enzymatic activity, mid-stage apoptosis, late-stage apoptosis, and oxidative stress.

Specifications

Assay Points
125 tests
Brand
ViaStain
Instrument Compatibility
Cellometer Spectrum Image Cytometry System
Quantity
1 kit
Shipping Conditions
Shipped Ambient
Storage Conditions
2-8°C, -16°C to -24°C
Type
Fluorescent Reagents and Assay Kits

Resources

Are you looking for resources, click on the resource type to explore further.

Scroll Icon