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PhenoVue Fluor 400LS - Concanavalin A

PhenoVue Fluor 400LS - Concanavalin A
HeLa cells stained with PhenoVue Fluor 400LS - Concanavalin A (endoplasmic reticulum). Imaged on Opera Phenix Plus system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 568 - Phalloidin, Fluor 488 - WGA, and Fluor 400LS Concanavalin A). Imaged on Opera Phenix Plus system. View All
PhenoVue Fluor 400LS - Concanavalin A
PhenoVue Fluor 400LS - Concanavalin A
HeLa cells stained with PhenoVue Fluor 400LS - Concanavalin A (endoplasmic reticulum). Imaged on Opera Phenix Plus system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 568 - Phalloidin, Fluor 488 - WGA, and Fluor 400LS Concanavalin A). Imaged on Opera Phenix Plus system.
PhenoVue Fluor 400LS - Concanavalin A
HeLa cells stained with PhenoVue Fluor 400LS - Concanavalin A (endoplasmic reticulum). Imaged on Opera Phenix Plus system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 568 - Phalloidin, Fluor 488 - WGA, and Fluor 400LS Concanavalin A). Imaged on Opera Phenix Plus system.
PhenoVue Fluor 400LS - Concanavalin A
PhenoVue Fluor 400LS - Concanavalin A
HeLa cells stained with PhenoVue Fluor 400LS - Concanavalin A (endoplasmic reticulum). Imaged on Opera Phenix Plus system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 568 - Phalloidin, Fluor 488 - WGA, and Fluor 400LS Concanavalin A). Imaged on Opera Phenix Plus system.
PhenoVue Fluor 400LS - Concanavalin A
HeLa cells stained with PhenoVue Fluor 400LS - Concanavalin A (endoplasmic reticulum). Imaged on Opera Phenix Plus system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 568 - Phalloidin, Fluor 488 - WGA, and Fluor 400LS Concanavalin A). Imaged on Opera Phenix Plus system.

PhenoVue Fluor 400LS - Concanavalin A is a long Stokes shift fluorescent dye conjugated lectin which displays high affinity for glycoproteins and glycolipids present at the cellular membranes. It can be used for cellular membrane staining, particularly the endoplasmic reticulum.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue Fluor 400LS - Concanavalin A exhibits bright orange fluorescence and can be easily multiplexed for imaging and high-content screening applications. It has been validated on Revvity's HCS instruments such as Operetta CLS and Opera Phenix Plus, and has a maximum excitation wavelength of 395 nm and a maximum emission wavelength of 585 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below. 

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View product information
Feature Specification
Color Orange
Filter Ex: 375-440 nm, Em: 550-650 nm
Fluorophore PhenoVue™ Fluor 400LS
Organelle and Cell Compartment Endoplasmic Reticulum
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PhenoVue Fluor 400LS - Concanavalin A is a long Stokes shift fluorescent dye conjugated lectin which displays high affinity for glycoproteins and glycolipids present at the cellular membranes. It can be used for cellular membrane staining, particularly the endoplasmic reticulum.

Part of Revvity's portfolio of cellular imaging reagents, PhenoVue Fluor 400LS - Concanavalin A exhibits bright orange fluorescence and can be easily multiplexed for imaging and high-content screening applications. It has been validated on Revvity's HCS instruments such as Operetta CLS and Opera Phenix Plus, and has a maximum excitation wavelength of 395 nm and a maximum emission wavelength of 585 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below. 

Click to copy promo code to clipboard.
March lab savings - buy on Revvity.com or Revvity punchout to SAVE 10% with promo code!
MARCH10
Sale ends 3/24. Terms and conditions apply.
View product information
Product Variant
Quantity: 2 x 1 mg
Part #:
CP94001
List Price
USD 385.35
Your online price:
For research use only. Not for use in diagnostic procedures.
Total list price:
USD 385.35
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PhenoVue Fluor 400LS - Concanavalin A
HeLa cells stained with PhenoVue Fluor 400LS - Concanavalin A (endoplasmic reticulum). Imaged on Opera Phenix Plus system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 568 - Phalloidin, Fluor 488 - WGA, and Fluor 400LS Concanavalin A). Imaged on Opera Phenix Plus system.
PhenoVue Fluor 400LS - Concanavalin A
HeLa cells stained with PhenoVue Fluor 400LS - Concanavalin A (endoplasmic reticulum). Imaged on Opera Phenix Plus system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 568 - Phalloidin, Fluor 488 - WGA, and Fluor 400LS Concanavalin A). Imaged on Opera Phenix Plus system.
PhenoVue Fluor 400LS - Concanavalin A
PhenoVue Fluor 400LS - Concanavalin A
HeLa cells stained with PhenoVue Fluor 400LS - Concanavalin A (endoplasmic reticulum). Imaged on Opera Phenix Plus system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 568 - Phalloidin, Fluor 488 - WGA, and Fluor 400LS Concanavalin A). Imaged on Opera Phenix Plus system.
PhenoVue Fluor 400LS - Concanavalin A
HeLa cells stained with PhenoVue Fluor 400LS - Concanavalin A (endoplasmic reticulum). Imaged on Opera Phenix Plus system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 568 - Phalloidin, Fluor 488 - WGA, and Fluor 400LS Concanavalin A). Imaged on Opera Phenix Plus system.
PhenoVue Fluor 400LS - Concanavalin A
PhenoVue Fluor 400LS - Concanavalin A
PhenoVue Fluor 400LS - Concanavalin A
HeLa cells stained with PhenoVue Fluor 400LS - Concanavalin A (endoplasmic reticulum). Imaged on Opera Phenix Plus system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 568 - Phalloidin, Fluor 488 - WGA, and Fluor 400LS Concanavalin A). Imaged on Opera Phenix Plus system.
PhenoVue Fluor 400LS - Concanavalin A
HeLa cells stained with PhenoVue Fluor 400LS - Concanavalin A (endoplasmic reticulum). Imaged on Opera Phenix Plus system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 568 - Phalloidin, Fluor 488 - WGA, and Fluor 400LS Concanavalin A). Imaged on Opera Phenix Plus system.

Product information

Overview

Concanavalin A is a plant homotetrameric lectin known to activate the immune system or induce apoptosis and autophagy. Concanavalin A displays high affinity for α-mannopyranosyl and α-glucopyranosyl residues of glycoproteins and glycolipids present at the cellular membranes.

Fluorescent Concanavalin A derivatives are commonly used for staining the cellular membranes of mammalian cells, particularly the endoplasmic reticulum.

PhenoVue Fluor 400LS - Concanavalin A can be used to visualize cellular membranes in immunofluorescence as well as high-content analysis and screening applications.

​​Its unique photophysical property enables extended multiplexing capability by adding a fifth color while ensuring no spectral overlap, high brightness as well as photostability.

Quantity or Volume Per Vial: 1mg (9.62 nmoles)

Recommended Working Concentration: 50 µg/mL

Equivalent Number of Microplates:

  • 1 - 4 x 96-well microplates
  • 1 - 4 x 384-well microplates
  • 2 - 6 x 1536-well microplates

Specifications

Color
Orange
Form
Lyophilized
Maximum Emission Wavelength (Emmax)
585 nm
Maximum Excitation Wavelength (Exmax)
395 nm
Application
High Content Imaging
Microscopy
Brand
PhenoVue™
Detection Modality
Fluorescence
Filter
Ex: 375-440 nm, Em: 550-650 nm
Fluorophore
PhenoVue™ Fluor 400LS
Organelle and Cell Compartment
Endoplasmic Reticulum
Quantity
2 x 1 mg
Sample Type
Live and fixed samples
Shipping Conditions
Shipped Ambient
Storage Conditions
2-8 °C, protected from light
Type
Individual Reagent

Image gallery

PhenoVue Fluor 400LS - Concanavalin A
HeLa cells stained with PhenoVue Fluor 400LS - Concanavalin A (endoplasmic reticulum). Imaged on Opera Phenix Plus system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 568 - Phalloidin, Fluor 488 - WGA, and Fluor 400LS Concanavalin A). Imaged on Opera Phenix Plus system.
PhenoVue Fluor 400LS - Concanavalin A
HeLa cells stained with PhenoVue Fluor 400LS - Concanavalin A (endoplasmic reticulum). Imaged on Opera Phenix Plus system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 568 - Phalloidin, Fluor 488 - WGA, and Fluor 400LS Concanavalin A). Imaged on Opera Phenix Plus system.
PhenoVue Fluor 400LS - Concanavalin A
PhenoVue Fluor 400LS - Concanavalin A
HeLa cells stained with PhenoVue Fluor 400LS - Concanavalin A (endoplasmic reticulum). Imaged on Opera Phenix Plus system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 568 - Phalloidin, Fluor 488 - WGA, and Fluor 400LS Concanavalin A). Imaged on Opera Phenix Plus system.
PhenoVue Fluor 400LS - Concanavalin A
HeLa cells stained with PhenoVue Fluor 400LS - Concanavalin A (endoplasmic reticulum). Imaged on Opera Phenix Plus system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 568 - Phalloidin, Fluor 488 - WGA, and Fluor 400LS Concanavalin A). Imaged on Opera Phenix Plus system.
PhenoVue Fluor 400LS - Concanavalin A
PhenoVue Fluor 400LS - Concanavalin A
HeLa cells stained with PhenoVue Fluor 400LS - Concanavalin A (endoplasmic reticulum). Imaged on Opera Phenix Plus system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 568 - Phalloidin, Fluor 488 - WGA, and Fluor 400LS Concanavalin A). Imaged on Opera Phenix Plus system.
PhenoVue Fluor 400LS - Concanavalin A
HeLa cells stained with PhenoVue Fluor 400LS - Concanavalin A (endoplasmic reticulum). Imaged on Opera Phenix Plus system.
5-plex experiment. HeLa cells stained with five PhenoVue stains (641 mitochondrial stain, Hoechst 33342 nuclear stain, Fluor 568 - Phalloidin, Fluor 488 - WGA, and Fluor 400LS Concanavalin A). Imaged on Opera Phenix Plus system.

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Resources

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Technical Note Icon
Technical Note
Expanding imaging multiplexing capabilities with PhenoVue Fluor 400LS - Phalloidin

This technical note offers tips and best practices for using PhenoVue® Fluor 400LS Phalloidin stain to optimize fluorescent signal...

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PhenoVue Cellular Imaging Reagents Brochure
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PhenoVue Cellular Imaging Reagents Flyer

This flyer describes Revvity's PhenoVue cellular imaging reagents.

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Product Info
PhenoVue Fluor - Concanavalin A Conjugates Product Information Sheet

Fluorescent Concanavalin A conjugates represent a method of choice for labelling the cellular membranes of mammalian cells...

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PhenoVue Reagents & Imaging Microplates Product List
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