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  • Who we serve
    • Academia
      Explore BioLegend

      Learn about our world class antibodies for a diverse set of research areas including immunology, neuroscience, cancer, stem cells and cell biology.

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  • Products
    • Research & Development
      • Genomic Analysis
        • IncRNA in Functional Studies
        • Nucleic Acid Isolation
        • NGS Workflows
        • Microplate Readers
        • Microfluidic Nucleic Acid Analysis
        • CRISPR Technologies
        • RNA Interference
        • Transfection and Ancillary Reagents
        • Oligonucleotide Custom Synthesis
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        • Viral Vector Products
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      • Protein Analysis
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        • Western Blotting
        • Sample Dissociation
        • M-PVA Magnetic Beads
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      • Cell Analysis
        • Cell Isolation
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        • Microplates
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      • Research Solutions
        • Immunoassays Solutions
        • pHSense Reagents
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        • Biologics
        • Small Molecule Drug Discovery
        • Disease Research
        • Target Class
        • Drug Development
        • Precision Medicine Research
        • Functional Genomic Screening Solutions
        • Assay Development Workflows
        • Microphysiological Systems
        • Biobanking Workflows
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    • Clinical & Diagnostics
      • Reproductive Health
        • Maternal and Prenatal Testing
        • Revvity Genomics Software
        • Newborn Screening
        • Newborn Sequencing Research
        • Pregnancy-Relevant Infections
        • Endocrine Reproductive
        • Cell-free DNA Analysis
        • Neonatal Research
        • Preimplantation Genetic Testing
        • PlGF Testing Research
        • Molecular Cytogenetics
        New IVD Mimix™ reference standards for oncology workflows.

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      • Infectious Diseases
        • Tuberculosis Management
        • EUROIMMUN Solutions
        • IDS Solutions
        • Nucleic Acid Isolation for Pathogen Detection
        • Cytomegalovirus
        • SARS-COV-2 Testing Solutions
        • Bacterial & Viral Nucleic Acid Isolation
        • Metagenomics
        New IVD Mimix™ reference standards for oncology workflows.

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      • Cancer
        • ASR Flow Cytometry Antibodies
        • HPV Testing
        • ctDNA Workflows
        • miRNA-seq Analysis
        • Exosome/cfRNA Analysis
        • Targeted Sequencing
        • Mimix Reference Standards
        • Functional Testing
        NEW! Mimix IVD reference standards

        Providing diagnostic labs with trusted quality controls for developing tests and monitoring workflows, with the added assurance of IVD marking. Explore our newest MimixTM controls.

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      • Autoimmunity
        • EUROIMMUN Solutions
        • IDS Solutions
        New IVD Mimix™ reference standards for oncology workflows.

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      • Endocrinology
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      • Allergy
        New IVD Mimix™ reference standards for oncology workflows.

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      • Neurodegeneration
        • Alzheimer's Disease
        • Amytrophic Lateral Sclerosis (ALS)
        • Neurogranin
        • BACE1
        New IVD Mimix™ reference standards for oncology workflows.

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      • Rapid Patient Testing
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      New IVD Mimix™ reference standards for oncology workflows.

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    • Reagents
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      • Cellular Imaging Reagents
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      • chemagic Nucleic Acid Purification Kits
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      • CRISPR Technologies
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      • Immunoassays
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      • Liquid Scintillation Cocktails
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      • pHSense Reagents
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      • qPCR
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    • Platforms & Automation
      • Nucleic Acid Purification
        • chemagic Applications
        • chemagic IVD Instruments
        • chemagic IVD Kits
        • chemagic Instruments
        • chemagic Kits
        • M-PVA Magnetic Beads
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Automated Liquid Handling
        • Flow Cytometry Cocktail Prep Automation
        • Liquid Handling Workstations
        • Automated Liquid Handling Applications
        • Liquid Handling Consumables & Accessories
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Integrated Lab Automation
        • Automated Plate Loading
        • Workstation Modules
        • Scheduling & Control Software
        • Robotics
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Microfluidic Analysis
        • Microfluidic Protein Characterization
        • Microfluidic Nucleic Acid Analysis
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Detection Solutions
        • Plate Reader Accessories
        • Microplate Readers
        • Radiometric Detectors
        • Radiochemicals
        • Liquid Scintillation Cocktails
        • Radiometric Consumables & Accessories
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Imaging
        • Cellular Imaging & Analysis
        • In Vivo Imaging
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Cell Counting and Image Cytometry
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Sample Homogenization
        • Homogenizers
        • Homogenizer Accessories
        • Homogenizer Consumables
        • Sample Centrifugation
        • Sample Sonication
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • In Vitro Diagnostics (IVD) Platforms & Automation
        • IVD Nucleic Acid Isolation
        • IVD Automated Liquid Handling
        • IVD Radiometric Detectors
        • EUROIMMUN Instruments
        • IDS Instruments
        • Clinical Applications
        New! Cellometer™ Ascend™

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      • Consumables & Accessories
        • Cassettes
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        • High-Content Imaging Accessories
        • Microplates
        • Radioactive Spill Cleaners
        • Scintillation Cocktails
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        • Charcoal Traps
        • Homogenizer Accessories
        • Homogenizer Consumables
        New! Cellometer™ Ascend™

        Explore our cell counting and image cytometry solutions.

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      • Instrument Service & Maintenance
        • On Demand Equipment Service
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        New! Cellometer™ Ascend™

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      New! Cellometer™ Ascend™

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    • Consumables & Accessories
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    • Signals Software
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        • ChemDraw
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        • Signals Notebook
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      • All Solutions
        • Biology
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        • Clinical & Translational
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  • Services
    • Preclinical Services
      • Antibody Drug Conjugate Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Complex Cell Model Screening Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Base Editing Platform
        • Pin-point base editing platform cell line engineering services
        • Pin-point base editing platform pooled tiled screening services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Immune Cell Screening
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Functional Genomic Screening Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Cell Panel Screening
        Preclinical services

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      • Cell Line Engineering
        • CHOSOURCE CLE Services
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      • Viral Vector Engineering and Manufacture
        Preclinical services

        Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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      Preclinical services

      Work with our experienced scientific team and leverage our advanced technologies to help accelerate the preclinical drug discovery process.

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    • Revvity Omics Services
      • Revvity Omics Clinical Services
        • Cytogenomics
        • Global Laboratory Network
        • Metabolic Testing
        • Newborn Screening Services
        • Prenatal Screening Services
        • Proactive Testing
        • Rare Disease Testing
        • Specialized and Customized Assays
        • Sponsored Testing Programs
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Revvity Omics Pharma Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Clinical & Testing Services
      • Revvity Omics Clinical Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Revvity Omics Pharma Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Cellular and Humoral Immunoassays
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Tuberculosis Testing Services
        • Oxford Diagnostic Laboratories Service Closures
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Customization Services
      • Assays and Reagents
        • Assay Development
        • Compound profiling
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Microplate Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Custom Conjugation & Labeling
        • Custom Labeling
        • Small Molecule
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Radiosynthesis and Labeling
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Licensing
      • Gene Delivery Licensing
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      • Gene Expression Systems
        • AAV Production Platform
        • CHOSOURCE Platform
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      • Pin-point™ Base Editing Platform
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      • Virus Screening
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      CHOSOURCE expression platform

      Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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    • Viral Vector Engineering and Manufacture
      • AAV Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Lentivirus Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Instrument Service & Maintenance
      • AV Services
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Equipment Service Plans
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • On-demand Equipment Service
        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Customer Training
      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • OEM Solutions
      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    T-SPOT.TB testing services.

    Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      Investor Day 2024

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  • Brands
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    • BioLegend

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    • Popular Revvity Product Brands
    • AlphaLISA and SureFire Ultra

    • BioQule NGS

    • chemagic

    • EnVision Nexus

    • Fontus

    • HTRF

    • IVIS

    • LEGENDplex

    • Opera Phenix

    • T-SPOT

    • TotalSeq

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  • Welcome to Revvity: renowned brands and boundless innovation.

    Hearing the word "can't" is our call to action!

    We help scientists, researchers, and clinicians overcome the world's greatest health obstacles.

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    Featured brand: BioLegend

    Learn about our world-class antibodies for a diverse set of research areas including immunology, neuroscience, cancer, stem cells and cell biology.

    Visit BioLegend.com

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HTRF Human XIAP / BIR3 Binding Red Ligand
Revvity Vial from a Kit, Standard or Control lysate
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Revvity Vial from a Kit, Standard or Control lysate
Revvity Vial from a Kit, Standard, or Control lysate
Revvity Vial from a Kit, Standard or Control lysate
Revvity Vial from a Kit, Standard or Control lysate
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HTRF XIAP BIR3 Red Ligand can be used for affinity binding experiments in association with the HTRF XIAP BIR3 Binding kit to investigate cooperativity effects within PROTAC drug discovery.

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HTRF XIAP BIR3 Red Ligand can be used for affinity binding experiments in association with the HTRF XIAP BIR3 Binding kit to investigate cooperativity effects within PROTAC drug discovery.

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Product variant
Unit Size: 1 unit
Part #:
64XIAPFLRED
List price
USD 427.39
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For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
Total list price:
USD 427.39
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Revvity Vial from a Kit, Standard or Control lysate
Revvity Vial from a Kit, Standard or Control lysate
Video Play Button
HTRF Human XIAP / BIR3 Binding Red Ligand
Revvity Vial from a Kit, Standard or Control lysate
Video Play Button
Revvity Vial from a Kit, Standard or Control lysate
Revvity Vial from a Kit, Standard, or Control lysate
HTRF Human XIAP / BIR3 Binding Red Ligand
Revvity Vial from a Kit, Standard or Control lysate
Revvity Vial from a Kit, Standard or Control lysate
Video Play Button
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Product information

  • Overview
  • How it works
  • Assay details
  • Assay validation
  • Specifications
  • Video gallery

Overview

The HTRF XIAP BIR3 Red Ligand is based on labeled A410099.1 derivative and is primarily intended to perform cooperativity binding studies. It enables calculation of the affinity of the XIAP BIR3 Red Ligand for XIAP BIR3 protein in the presence of the targeted protein of interest. The Kd of HTRF XIAP BIR3 Red Ligand for XIAP protein, in absence and presence of the targeted protein, enables an accurate analysis of the cooperative effect assessed with the HTRF XIAP BIR3 binding kit.

How it works

HTRF XIAP ligand binding assay principle on the XIAP BIR3 binding domain

HTRF XIAP BIR3 Red Ligand binding is detected in a direct binding assay format, using an anti GST-Terbium Cryptate antibody which binds to GST-tagged Human XIAP BIR3 domain. When the dyes are in close proximity, the excitation of the donor with a light source (laser or flash lamp) triggers a Fluorescence Resonance Energy Transfer (FRET) towards the acceptor, which in turn fluoresces at a specific wavelength (665 nm) (Image A). The specific binding signal is calculated by subtracting the non-specific binding signal from the total signal, enabling Kd determination for the HTRF XIAP BIR3 Red Ligand (Image B).

ppi-how-it-works-assay-principle-human-xiap-bir3-red-ligand
ppi-how-it-works-assay-principle-human-xiap-bir3-red-ligand
HTRF XIAP BIR3 Red Ligand binding assay protocol

Saturation binding experiments of HTRF XIAP BIR3-Red Ligand can be run in 384-well plates by dispensing 5 µL of Diluent 9 buffer (for Total binding) or XIAP BIR3 Binding standard (for non specific binding). Then 5 µL of PROTAC Binding Buffer 2 (for reference) or the targeted protein diluted in PROTAC Binding Buffer 2 (for cooperativity studies) are added, followed by 5 µL of GST-tagged XIAP BIR3 protein. Finally, 5 µL of a pre-mixed solution of HTRF XIAP BIR3-Red Ligand and Anti-GST Tb cryptate antibody are added.

The HTRF Ratio is measured after 2 hours of incubation at room temperature

ppi-how-it-works-assay-protocol-human-xiap-bir3-red-ligand

 

Assay details

HTRF XIAP BIR3-Red Ligand Kd (reference without PROTAC protein substrate) 5 nM +/- 8nM

 

Assay validation

Determination of the affinity binding (Kd) of XIAP BIR3-Red Ligand in the presence of targeted protein

In this example, the affinity binding (Kd) of the XIAP BIR3 Red Ligand to GST-XIAP BIR3 protein was assessed in the absence or presence of the targeted protein BRD4 (10nM or 100nM)  . This result indicates that the presence of BRD4 does not change the Kd of the Red Ligand.

Consequently this example shows that a cooperativity experiment with a PROTAC molecule composed of a XIAP ligand and a BRD4 warhead can be set up. Finally, the alpha factor can be established by dividing the Ki of the PROTAC in the binary complex (XIAPBIR3 -PROTAC) by the Ki of the PROTAC in the ternary complex (XIAPBIR3 -PROTAC-BRD4).

assay-validation-human-xiap-bir3-red-ligand

 

Specifications

Automation Compatible
Yes
Brand
HTRF
Detection Modality
HTRF
Product Group
Fluorescent Reagent
Shipping Conditions
Shipped in Dry Ice
Technology
TR-FRET
Therapeutic Area
Inflammation
Neuroscience
Oncology & Inflammation
Unit Size
1 unit

Video gallery

HTRF Human XIAP / BIR3 Binding Red Ligand
HTRF Human XIAP / BIR3 Binding Red Ligand

Resources

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Guide Icon
Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.

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Guide
The ultimate guide to PPI assays with HTRF

Your dream assay does not exist yet? Create it!

This guide will provide you with tools to help you develop your very own protein...

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SDS, COAs, manuals and more

Are you looking for technical documents related to the product? We have categorized them in dedicated sections below. Explore now or request your COA/TDS, SDS, or IFU/manual.

Safety data sheet
  • Language
    English
    Country
    United States
    Name
    SDS Iconrvty_ls_sds_64XIAPFLRED_US.pdf
  • Language
    French
    Country
    France
    Name
    SDS Iconrvty_ls_sds_64XIAPFLRED_FRA.pdf
  • Language
    German
    Country
    Germany
    Name
    SDS Iconrvty_ls_sds_64XIAPFLRED_DEU.pdf
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Technical data sheet
  • Lot Number
    01RA
    Lot Date
    September 9, 2026
    Name
    SDS IconTechnical Data Sheet : HTRF XIAP BIR3 Binding Kit Red Ligand / 64XIAPFLRED
  • Lot Number
    01A
    Lot Date
    September 9, 2026
    Name
    SDS IconTechnical Data Sheet : HTRF XIAP BIR3 Binding Kit Red Ligand / 64XIAPFLRED
  • Lot Number
    01A
    Lot Date
    September 9, 2024
    Name
    SDS Iconrvty_ls_tds_20240909_64XIAPFLRED_01A.pdf
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IFUs, manuals & more
  • Resource Type
    Manual
    Language
    English
    Country
    -
    Name
    SDS IconManual : HTRF XIAP BIR3 Binding Kit Red Ligand / 64XIAPFLRED

Request an Request a Manual/IFU or Contact us for support regarding any other missing documentation like Barcodes, DOC, etc.

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