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DepleteX RNA Depletion (Globin)
DepleteX RNA Depletion (Globin)
Jumpcode-box-image_700x700
The DepleteX® Globin Depletion Kit selectively removes highly expressed globin transcripts (HBA1, HBA2, HBB, & HBD) in vitro before sequencing. This process redirects sequencing resources toward unique biologically relevant transcripts, maximizing UMI and gene sensitivity.
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| Feature | Specification |
|---|---|
| Automation Compatible | No |
| Product Group | Ribodepletion |
The DepleteX® Globin Depletion Kit selectively removes highly expressed globin transcripts (HBA1, HBA2, HBB, & HBD) in vitro before sequencing. This process redirects sequencing resources toward unique biologically relevant transcripts, maximizing UMI and gene sensitivity.
Click to copy promo code to clipboard.
Product Variant
Unit Size: 24 rxns
Part #:
NOVA-512870
List Price
USD 1,224.00
Your online price:
For research use only. Not for use in diagnostic procedures.
DepleteX RNA Depletion (Globin)
Jumpcode-box-image_700x700
DepleteX RNA Depletion (Globin)
Supporting products you might need
Do you need a RNA-seq library prep kit?
Product information
Overview
Enhance RNA-Seq quality by eliminating globin fragments.
Apart from ribodepletion, RNA samples often contain a limited number of well-characterized, highly expressed transcripts. However, resequencing these transcripts contributes minimally to biologically relevant information. The dominance of highly expressed transcripts hinders coverage for low-expressing counterparts, resulting in reduced sensitivity for detecting disease-related low-expressing genes, rare variants, and unique fusion events.
Globin mRNA dominates the mRNA content of red blood cells and whole blood. Three globlin genes (HBA1, HBA2 and HBB) constitute ≥30% of total mRNA from blood.
Key highlights:
- Unbiased removal of human globin from RNA-seq libraries
- Achieve >99% depletion of contaminating globin
- Rapid one-hour depletion incubation
Specifications
| Automation Compatible |
No
|
|---|---|
| Product Group |
Ribodepletion
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Unit Size |
24 rxns
|
Resources
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Technical Note
DepleteX™ Globin Depletion Kit
Remove contaminating globin fragments for RNA-Seq
Flyer
NEXTFLEX RNA-seq portfolio
This flyer illustrates the breadth of the NEXTFLEX RNA-seq Portfolio
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