Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-SLP76 (Tyr145) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-SLP76 (Tyr145) assay kit, but can also be ordered separately.
Feature | Specification |
---|---|
Application | Cell Signaling |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-SLP76 (Tyr145) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-SLP76 (Tyr145) assay kit, but can also be ordered separately.
This control lysate is intended for use with the AlphaLISA SureFire UltraHuman and Mouse p-SLP-76 (Tyr145) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, Raw264.7 cells were cultured to confluence in T175 flasks in 10% FBS containing medium. Cells were treated with 11 mM H2O2 and 50 nM Calyculin A for 10 minutes at 37°C. Cells were lysed in 5 mL of SureFire Ultra Lysis Buffer.
Application |
Cell Signaling
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Modality |
Alpha
|
Lysis Buffer Compatibility |
Lysis Buffer
|
Molecular Modification |
Phosphorylation
|
Product Group |
Control Lysate
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
SLP76
|
Target Class |
Phosphoproteins
|
Target Species |
Human
Mouse
|
Technology |
Alpha
|
Therapeutic Area |
Autoimmunity
Immuno-oncology
Inflammation
Neuroscience
|
Unit Size |
1 Vial
|
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