PhenoVue Live/Dead Cell Viability Assay Kit

PhenoVue Live/Dead Cell Viability Assay Kit provides a two-color green / red fluorescence method which enables the simultaneous determination of live and dead cells using two different dyes. Calcein AM dye penetrates live cells where intracellular esterases hydrolyze the dye to generate a bright green, fluorescent signal. Propidium Iodide dye enters damaged cell membranes of dead cells and undergoes a 40-fold enhancement of fluorescence upon binding to nucleic acid, thereby producing a bright red fluorescence. This assay kit provides an easy-to-use method for measuring cell proliferation, cell viability, cytotoxicity and apoptosis.

Kit Components / Quantity:

• Calcein-AM/ 2 vials - lyophilized
• Propidium Iodide / 1 vial - liquid
• DMSO / 1 vial – liquid
• Assay Buffer / 1 bottle - liquid

Equivalent number of microplates:

• 2 x 96-well microplates, or
• 2 x 384-well microplates, or
• 3 x 1536-well microplates