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AlphaLISA Surefire Ultra Mouse Phospho-RIP3 (Thr231/Ser232) Detection Kit, Control Lysate
AlphaLISA Surefire Ultra Mouse Phospho-RIP3 (Thr231/Ser232) Detection Kit, Control Lysate
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-RIP3 (Thr231/Ser232) (Mouse) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-RIP3 (Thr231/Ser232) (Mouse) assay kit, but can also be ordered separately.
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-RIP3 (Thr231/Ser232) (Mouse) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-RIP3 (Thr231/Ser232) (Mouse) assay kit, but can also be ordered separately.
AlphaLISA Surefire Ultra Mouse Phospho-RIP3 (Thr231/Ser232) Detection Kit, Control Lysate
AlphaLISA Surefire Ultra Mouse Phospho-RIP3 (Thr231/Ser232) Detection Kit, Control Lysate
Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Mouse Phospho-RIP3 (Thr231/Ser232) Assay Kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, L929 cells were cultured to confluence in T175 flasks in 10% FBS containing medium. Cells were treated with 50 ng/mL mTNFα, 20 μM Z-VAD and 200 nM SM-164 for 2 hours at 37°C. Cells were lysed in 5 mL of 1X SureFire Ultra Lysis Buffer.
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Control Lysate
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
RIP3
|
| Target Class |
Phosphoproteins
|
| Target Species |
Mouse
|
| Technology |
Alpha
|
| Therapeutic Area |
Autoimmunity
Central Nervous System
Neuroinflammation
|
| Unit Size |
1 Vial
|
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AlphaLISA Surefire Ultra Mouse Phospho-RIP3 (Thr231/Ser232) Detection Kit, Control Lysate
AlphaLISA Surefire Ultra Mouse Phospho-RIP3 (Thr231/Ser232) Detection Kit, Control Lysate
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