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Technical Note
Guidelines from PBMC isolation to cytokine assay optimization
This tech note will provide you with a complete step by step methodology to purify PBMCs and use them in cytokine quantification assays with HTRF
Technical Note
Optimize your HTRF cell signaling assays for tissues
This technical note gives precise guidelines for tissue lysate preparation, assay optimization, data analysis, and then interpretation, guaranteeing you will get accurate and interpretable results.
Technical Note
Optimization and pharmacological validation of wild type beta-arrestin 2 plasmid transfection
This technical note provides details on how transfecting cell lines with a beta-arrestin plasmid can lead to an increase in beta-arrestin 2 recruitment signals and restoration of beta-arrestin 2 in otherwise deficient cells.
Technical Note
Smart Tray accessories for higher throughput optical imaging on the IVIS Spectrum 2 and IVIS SpectrumCT 2
Using Smart Tray accessories for higher throughput optical imaging on the IVIS Spectrum 2 and IVIS SpectrumCT 2.
Technical Note
Key success tips to perform β-arrestin 2 recruitment assays by using the B-arr2 recruitment kit with GPCRs
This technical note provides valuable tips for performing B-arr2 recruitment assays on adherent cell lines overexpressing GPCRs.
Technical Note
Lysis buffer compatibility HTRF assays
This leaflet presents the lysis buffer compatibility for HTRF assays.
Technical Note
Guidelines for cell culture and lysis in different formats prior to HTRF detection
This Technical Note will provide you with guidelines on adapting the standard 96-well plate protocol for seeding and lysing cells to different culture formats such as T75 flasks, or 6-well, 12-well, and 24-well plates.
Technical Note
Comparison of cell concentration and viability using the Cellometer Auto 2000 cell counter and a manual hemocytometer.
Technical note comparing measurement of cell concentration and viability using the Cellometer Auto 2000 cell counter and a manual hemocytometer.
Catalog
Technical Note
Expanding imaging multiplexing capabilities with PhenoVue Fluor 400LS - Phalloidin
This technical note offers tips and best practices relating to fluorescent signal quality using PhenoVue Fluor 400LS Phalloidin stain.
Technical Note
AlphaLISA Acetyl‑Histone H3 lysine 9 (H3K9ac) cellular assay
This technical note provides information on how the AlphaLISA immunodetection assay detects variations in acetylated histone H3 lysine 9 (H3K9ac) levels within cellular extracts
Technical Note
AlphaLISA DOT1L histone H3 lysine-N-methyltransferase assay
This Technical Note will provide you with guidelines on the optimization of a DOT1L enzymatic assay using oligonucleosomes as the substrate
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