PhenoVue Cell Painting Kits

Cell Painting is a powerful and new phenotypic high-content screening approach which combines cell and computational biology to unravel cells responses when subjected to perturbagens, such as chemical compounds, or gene expression modulators.

In this process, cells are “painted” by labeling various cellular compartments with different fluorescent bioprobes to quantitatively profile multiple phenotypic parameters to better understand the effects of chemical compounds, drugs, genes, or other test articles. Cell compartments and organelles are simultaneously tagged with six fluorescent probes, followed by acquisition and analysis of images. The six probes target specific cell compartments to determine protein expression or signaling pathways, identify organelles and their function, or identify whole-cell morphology.

It is recommended that researchers follow the protocols described in the Bray et al. (2016) publication or published by the JUMP consortium. Revvity PhenoVue cell painting kits (two versions available) have been designed to match the experimental conditions described and comprise validated, preoptimized fluorescent bioprobes to streamline workflows, saving you time and cost. Each kit contains all the necessary cell painting reagents (stains/fluors and diluent) as well as a step-by-step protocol to maximize your cell painting assay efficiency. The protocols are for use with 384-well microplates but can easily be adapted for use with 96-well microplates.

Proud to be supporting the JUMP-CP consortium

We are proud to be supporting the Joint Undertaking in Morphological Profiling-Cell Painting (JUMP-CP) consortium, providing our PhenoVue Cell Painting JUMP kits to the Consortium to create the world’s largest, public cell painting dataset. See our press release in the Resources tab for more details.

References:

1.  Bray M, Singh S, Han H, Davis C, Borgeson B, Hartland C et al. Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes. Nature Protocols. 2016;11(9):1757-1774.
2.  JUMP-Cell Painting Consortium [Internet]. Jump-cellpainting. broadinstitute.org. [cited 01 December 2022]. Available from: https://jump-cellpainting.broadinstitute.org/. Preprint available at: https://www.biorxiv.org/content/10.1101/2022.07.13.499171v1