GrowDex Hydrogels

More than ever, researchers are turning to 3D cell cultures, microtissues and organoids to bridge the gap between 2D cell cultures and in vivo animal models. Our solutions-based approach is designed to support you at every step of the cell culture workflow so you can seamlessly culture, treat, and analyze 3D cell cultures. Begin generating more physiologically relevant data to better understand biological processes and make informed decisions with our solutions. For research use only. Not for use in diagnostic procedures

Here we establish methodologies for 3D cell culture of the common neuronal cell line SH-SY5Y within the GrowDex® hydrogel to provide a more biomimetic set of environmental cues with which to promote cell migration and neurite outgrowth, whilst sustaining the viability of the population.

Here, adipose derived MSCs (ADMSCs) were cultured in nanofibrillar cellulose, GrowDex®, under various conditions for 1 week to characterise their behaviour ahead of use in the development of further cell based assays.

Neuronal ceroid lipofuscinosis (NCL) is a complex, rare, and fatal disease belonging to a larger group of lysosomal storage disorders that presents with nervous system symptoms. Batten disease – or CLN3 disease – is another name given commonly to Juvenile NCL (JNCL). Dr. Gomez-Giro and her team focused on understanding the neurodevelopmental process of JNCL utilizing healthy hiPSCs introduced with a disease-causing c.1054C→T pathologic variant into the CLN3 gene. Read the whitepaper to find out more about Dr. Gomez-Giro's research.

When working with 3D cell models, high heterogeneity between samples can pose a challenge - either biological or technical. In this application note, we demonstrate how a JANUS G3 liquid handling workstation can be used to improve throughput as well as assay statics by automating the liquid handling steps of 384-well, hydrogel-based high-content toxicity assay.

In this study, the morphology development of MCF7 cells in wood-derived nanofibrillar cellulose hydrogel GrowDex® (3D), and in mouse tumor basement membrane extract (BME) Matrigel overlay culture (2.5D) are presented.

'In this study diffusion rates of small drug molecules, namely nadolol, ketoprofen and metronidazole, through GrowDex-T were evaluated.

In our application note, we evaluate the compatibility of GrowDex, a wood-derived nanofibrillar cellulose hydrogel 3D cell culture matrix , with Revvity’s HTRF and AlphaLISA SureFire ® Ultra ™ lysis buffers and cell-based assay kits, using cell lysate samples from MCF7 3D cell cultures. The results demonstrate that HTRF and AlphaLISA cell lysis buffers effectively lyse MCF7 3D cells embedded and cultured in GrowDex. The effectiveness of different lysis buffers is confirmed by measuring total cellular protein using the BCA protein kit, as well as the expression and phosphorylation of Retinoblastoma protein pRb using HTRF or AlphaLISA SureFire Ultra kits. For research use only. Not for use in diagnostic procedures.

This data sheet provides instructions for use for GrowDase cellulase enzyme.

This data sheet provides instructions for use for GrowDex hydrogel.

This is a guide for selecting the right GrowDex hydrogel for your application.

This guide describes a number of experimental protocols performed using GrowDex hydrogels.

Here we describe how GrowDex-A can be functionalized with biotinylated proteins that provide cell adhesion motifs.

This data sheet provides instructions for use for GrowDex-A hydrogel.

This data sheet provides instructions for use for GrowDex-T hydrogel.

Here we establish methodologies for 3D cell culture of human iPSC-derived neural progenitors within nanofibrillar cellulose (NFC) hydrogel, GrowDex®-T.

Here we establish methodologies for 3D cell culture of human iPSC-derived neural progenitors within nanofibrillar cellulose (NFC) hydrogel, GrowDex®.

In this study pre-differentiated human pluripotent stem cell derived neurons where encapsulated within GrowDex and cultured.

Download our guide to reducing the costs and time required to set up reproducible high-throughput and high-content screens using 3D cell culture models. Topics include: Opportunities and challenges in 3D cell culture models for HTS and HCS Challenges of scaling up 3D HTS/HCS assays using animal-derived matrices Comparison of the costs and time required for running 3D cell culture HTS assays with animal-derived matrices and animal-free GrowDex hydrogel

Here we provide guidelines for using GrowDex® in cell-based assays.

Here we have started addressing some of these challenges using GrowDex® hydrogel as a 3D cell culture matrix for growing liver-derived HepG2 cells in 384 well microtiter plates.

Animal-derived matrices for 3D cell culture are often poorly defined and suffer from lot-to-lot variability. This variability ultimately affects the reproducibility of the experiment, which is an essential element for reliable research studies. Natural, reliable and ready-to-use, GrowDex ® is the next generation solution for reproducible and scalable animal-free 3D cell culture applications providing consistent results with convenience. GrowDex is produced from wood-derived nanofibrillar cellulose which guarantees its pure and clean structure as well as reproducible lots. Download our guide to learn how to transfer your 3D cell culture to GrowDex from animal-derived matrices.

In this study, our aim was to establish long-term tissue cultures from human biopsy pieces, and to evaluate the preservation of tissue-typical protein expressions

In this application note, 3D culture of novel NRAS mutated human melanoma cell line MUG-Mel2 in GrowDex® is presented.