Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-CHK2 (Thr68) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-CHK2 (Thr68) assay kit, but can also be ordered separately.
Feature | Specification |
---|---|
Application | Cell Signaling |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-CHK2 (Thr68) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-CHK2 (Thr68) assay kit, but can also be ordered separately.
This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-CHK2 (Thr68) Assay Kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, HEK293 cells were cultured to confluence in T175 flasks in 10% FBS containing medium, then treated with 5 μM Doxorubicin HCl for 30 minutes and lysed in 4 mL of 1X SureFire Ultra Lysis buffer.
Application |
Cell Signaling
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Modality |
Alpha
|
Lysis Buffer Compatibility |
Lysis Buffer
|
Molecular Modification |
Phosphorylation
|
Product Group |
Control Lysate
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
CHK2
|
Target Class |
Phosphoproteins
|
Target Species |
Human
|
Technology |
Alpha
|
Therapeutic Area |
Oncology
|
Unit Size |
1 Vial
|
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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