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The ease of colorimetric detection

For the convenience of direct colorimetric visualization of results without the need for a film or imaging instrument, count on our chromogenic substrates. With permanent results recorded on transfer membrane that won’t fade over time, chromogenic substrates provide an easy and cost-effective technique for western blotting.

For detection of horseradish peroxidase (HRP) use our 4CN Plus or DAB substrate solutions. For sensitive detection of alkaline phosphastase (AP), use our ready to use single component BCIP/NBT substrate.


For research use only. Not for use in diagnostic procedures.

Chromogenic Substrates
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DAB Substrate Solution

DAB Substrate Solution 10ml concentrate Shipped ambient.

Part Number: NEL938001EA
List price:
USD 148.04
BCIP/NBT Chromogenic Substrate

BCIP/NBT substrate detects alkaline phosphatase in blotting and slide applications. Available in ready to use solution, it deposits a permanent dark purple stain on membrane sites bearing phosphatase and with the combination of BCIP(5-bromo-4-chloro-3indolyl-phosphate and NBT(nitrobluetetrazoleum) produces much higher sensitivity than either reagent separately.

Part Number: NEL937001PK
List price:
USD 214.06
4CN Plus Chromogenic Substrate

4CN Plus substrate detects HRP and is ideal for western blotting applications. It produces dark purple precipitate in the prescence of HRP and is 10 times more sensitive than standard 4CN(4-chloro-1-napthol) formulations.

Part Number: NEL300001EA
List price:
USD 295.00
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Western blotting solutions

With applications in biotechnology, molecular biology, proteomics, and more, western blotting is the most widely used technique for detecting protein expression levels in cells. Our wide range of western blotting solutions gives you high levels of sensitivity, specificity, and selectivity so you can be confident in your results. Our solutions include: Transfer membranes Primary & Secondary antibodies Chemiluminescent substrates Chromogenic substrates