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      • Microfluidic Analysis
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  • Services
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      • Complex Cell Model Screening Services
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      • Base Editing Platform
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      • Immune Cell Screening
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      • Revvity Omics Pharma Services
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        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Clinical & Testing Services
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        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Cellular and Humoral Immunoassays
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        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Customization Services
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        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Custom Conjugation & Labeling
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        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      • Radiosynthesis and Labeling
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        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    • Licensing
      • Gene Delivery Licensing
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      • Gene Expression Systems
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        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      • Pin-point™ Base Editing Platform
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      • Virus Screening
        CHOSOURCE expression platform

        Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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      CHOSOURCE expression platform

      Revvity's expression platform provides an enhanced system for the development and manufacturing of biotherapeutics that can be used in commercial manufacturing applications.

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    • Viral Vector Engineering and Manufacture
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        T-SPOT.TB testing services.

        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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        Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      T-SPOT.TB testing services.

      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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      Revvity's Oxford Diagnostic Laboratories is a large referral laboratory for tuberculosis testing services based on our T-SPOT technology.

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    T-SPOT.TB testing services.

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    Learn about our world-class antibodies for a diverse set of research areas including immunology, neuroscience, cancer, stem cells and cell biology.

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HTRF Dasatinib Red Ligand, 20 nmoles
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Dasatinib-Red is a derivative of Dasatinib, and has been validated as a fluorescent tracer for HTRF Kinase Binding assays.

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Feature Specification
Application Biochemical Enzymatic Assay
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Dasatinib-Red is a derivative of Dasatinib, and has been validated as a fluorescent tracer for HTRF Kinase Binding assays.

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Product variants
Unit Size: 1 nmol
Part #:
62KB02REDC
List price
USD 1,003.30
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Unit Size: 20 nmol
Part #:
62KB02REDE
List price
USD 14,103.14
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For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
Total list price:
USD 14,103.14
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Revvity Vial from a Kit, Standard or Control lysate
Revvity Vial from a Kit, Standard or Control lysate
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HTRF Dasatinib Red Ligand, 20 nmoles
Revvity Vial from a Kit, Standard or Control lysate
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Revvity Vial from a Kit, Standard or Control lysate
Revvity Vial from a Kit, Standard, or Control lysate
HTRF Dasatinib Red Ligand, 20 nmoles
Revvity Vial from a Kit, Standard or Control lysate
Revvity Vial from a Kit, Standard or Control lysate
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Product information

  • Overview
  • How it works
  • Assay validation
  • Specifications
  • Video gallery

Overview

This compound is derived from Dasatinib, which is a well known inhibitor of the BCR-ABL and SRC families of Kinases. Dasatinib, which is an ATP-competitive Kinase inhibitor, is used as a drug to treat chronic myelogenous leukemia and acute lymphoblastic leukemia. It has been shown to bind with an affinity of <100 nM to about 16% of the Kinases and mainly to RTK. It is therefore useful to address multiple Kinases in the HTRF Kinase Binding assay platform. The ‘d2’ fluorescent dye used absorbs with its maximum near 650 nm and fluoresces near 670 nm. It is used as an acceptor molecule in HTRF assays.

Dasatinib-Red is supplied as a 25 µM solution in DMSO.

How it works

Kd determination: assay principal

​The binding of Dasatinib-Red is detected in a sandwich assay format using the Anti Tag labeled with Europium Cryptate (donor) which binds to the tagged Kinase, and a red fluorescent tracer labelled with d2 (acceptor).
The detection principle is based on HTRF technology.  The HTRF ratio (665/620) will increase upon the addition of more of the tracer, and will saturate depending on the dissociation constant (Kd) of the tracer to the tagged kinase. 
To know whether Dasatinib-Red is the tracer best suited to your tagged kinase of interest, we advise you to try the Kinase Binding Discovery kits.

1kinase-binding-how-it-works-assay-principal-saturation-staurosporine-red-62kb01redc-62kb01rede.svg

 

Kd determination assay protocol

Saturation binding experiments of  Dasatinib-Red can be run in 96- or 384-well plates (20 µL final volume).
First, a dilution series ranging between 0 and 1  µM of Dasatinib-Red in the Kinase Binding Buffer is prepared in a 96-well non-binding plate. Next, 5 µL of Kinase Binding Buffer are dispensed into the final 96- or 384-well plate. Then 5 µL of tagged-Kinase are added, followed by 5 µL of Anti-tag Eu-cryptate. Finally, 5 µL of the red tracer solution are added. 
The HTRF ratio is measured after 1 H of incubation.

2kinase-binding-how-it-works-assay-protocol-saturation-staurosporine-red-62kb01redc-62kb01rede.svg
 
IC50/Ki determination assay principle

The binding of Dasatinib-Red is detected in a sandwich assay format using a specific Anti-GST, 6HIS antibody or Streptavidin labeled with Europium Cryptate (donor), which binds to the tagged Kinase, and a red fluorescent Dasatinib labelled with d2 (acceptor).
The detection principle is based on HTRF® technology.  The HTRF ratio (665/620) will increase upon the addition of more Dasatinib-Red, and will saturate depending on the dissociation constant (Kd ) of Staurosporine-Red to the tagged kinase. With the addition of an inhibitor of the kinase, Dasatinib-Red will be displaced and the HTRF signal will disappear, depending on the dose.

3kinase-binding-how-it-works-assay-principal-inhibition-staurosporine-red-62kb01redc-62kb01rede.svg

 

IC50/Ki determination assay protocol

​​Pharmacological evaluation of inhibitors of interest can be run in 96- or 384-well plates.

First, a dilution series of inhibitor ranging between 40 µM and 0.23 nM is prepared, wih 5 µL of each concentration dispensed into the plate. Then 5 µL of tagged-Kinase are added, followed by 5 µL of anti-tag Eu-cryptate. Finally, 5 µL of Dasatinib-Red solution are added, prepared at 4x the final concentration.  The HTRF ratio is measured after 1 H of incubation.

Analyses of the data give typical dose response curves ranging between 10 µM and 56 pM, enabling evaluation of the IC50/Ki values for the inhibitor of interest.

4kinase-binding-how-it-works-assay-protocol-inhibition-staurosporine-red-62kb01redc-62kb01rede.svg

 

 

Assay validation

Saturation Binding (Kd)

Before perfoming competitive binding studies of inhibitors, the dissociation constant ​(Kd) of Dasatinib-Red has to be determined on the tagged Kinase of interest. This can be done with the help of the Kinase-GST/6HIS/biotin Discovery kits. A typical saturation binding experiment is performed using tracer concentrations between 0 and 250 nM, and measuring total- and non-specific binding signals. Subtracting the non-specific from the total binding signal gives the specific signal, which can be analysed to give the Kd. Here an example is shown on the determination of the Kd (22 nM) of Dasatinib-Red on 5 nM BRAF-GST. ​

1assay-validation-kinase-binding-dasatinib-red-1.svg

 

Competitive Binding (IC50/Ki)

Dose response curves of various known kinase inhibitors (Staurosporine, Dasatinib, PP2, Imatinib, Tozasertib, Sunitinib, Gefitinib, and Sorafenib) were measured using Dasatinib-Red at its Kd (22 nM) on 5 nM BRAF-GST. Dasatinib and Sorafenib showed good potencies, in good correlation with literature values.​​ Staurosporine, Tozasertib, Sunitib and Gefinitib did not compete, as can be expected for BRAF.

2assay-validation-kinase-binding-dasatinib-red-2.svg

 

Specifications

Application
Biochemical Enzymatic Assay
Brand
HTRF
Detection Modality
HTRF
Product Group
Fluorescent Reagent
Shipping Conditions
Shipped in Dry Ice
Target Class
Kinases
Technology
TR-FRET
Therapeutic Area
Metabolism/Diabetes
Neuroscience
Oncology & Inflammation
Unit Size
20 nmol

Video gallery

HTRF Dasatinib Red Ligand, 20 nmoles
HTRF Dasatinib Red Ligand, 20 nmoles

Resources

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Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.

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SDS, COAs, manuals and more

Are you looking for technical documents related to the product? We have categorized them in dedicated sections below. Explore now or request your COA/TDS, SDS, or IFU/manual.

Safety data sheet
  • Language
    English
    Country
    United States
    Name
    SDS Iconrvty_ls_sds_62KB02REDE_US.pdf
  • Language
    French
    Country
    France
    Name
    SDS Iconrvty_ls_sds_62KB02REDE_FRA.pdf
  • Language
    German
    Country
    Germany
    Name
    SDS Iconrvty_ls_sds_62KB02REDE_DEU.pdf
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Unable to find the relevant SDS? Try Searching or Request an SDS

Technical data sheet
  • Lot Number
    05A
    Lot Date
    October 7, 2026
    Name
    SDS IconTechnical Data Sheet : HTRF Dasatinib-Red / 62KB02REDE
  • Lot Number
    05RA
    Lot Date
    October 7, 2026
    Name
    SDS IconTechnical Data Sheet : HTRF Dasatinib-Red / 62KB02REDE
  • Lot Number
    05A
    Lot Date
    October 7, 2024
    Name
    SDS Iconrvty_ls_tds_20241007_62KB02REDE_05A.pdf
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IFUs, manuals & more
  • Resource Type
    Manual
    Language
    English
    Country
    -
    Name
    SDS IconManual : HTRF Dasatinib-Red / 62KB02REDC-62KB02REDE

Request an Request a Manual/IFU or Contact us for support regarding any other missing documentation like Barcodes, DOC, etc.

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