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Application Note

Rapid no wash assays for characterizing a mouse TIGIT/PD-L1 bispecific antibody

Technical advancements in antibody engineering has brought about greater interest in more novel antibody therapeutic design and the emergence of new classes of antibody therapeutics called bispecific antibodies (bsAbs). The principle behind bispecific antibody design is to create an antibody / antibody fragment to two or more binding sites to help with the treatment of complex diseases.

As more bsAbs are produced as therapeutics, fast and accurate methods for functionally evaluating and characterizing the stability of these antibodies are necessary during both discovery and development stages, as well as during formulation and quality analysis.

In this application note, we demonstrate how AlphaLISA assay technology with the EnVision multimode plate reader can be used for bispecific antibody detection, through an example application to characterize the binding and specificity of a mouse bispecific antibody targeting mouse TIGIT and mouse PD-L1.

You will find out:

  • What bispecific antibodies are and how they differ from classical antibody formats
  • How to design quick and easy experiments to measure binding of the bispecific antibody to the target proteins
  • How a no-wash multiplex assay can show direct binding of each site to its respective target simultaneously in one well
  • How these assays could be used to evaluate the stability and functional reproducibility between batches of the bsAb preparations

For research use only. Not for use in diagnostic procedures.

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Rapid no wash assays for characterizing a mouse TIGIT/PD-L1 bispecific antibody

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