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Optimizing 384-well qPCR plate preparation while decreasing costs

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Quantitative Polymerase Chain Reaction (qPCR) is an important step in many laboratory experiments to accurately quantify nucleic acids in next generation sequencing (NGS) and other applications. Propagation of errors from inaccurate liquid transfers can greatly impact the quality of a qPCR assay.

The Centre for Cancer Biology (SA Pathology and the University of South Australia) compared multiple ways of preparing qPCR plates using:

  • Manual Preparation
  • JANUS® G3 Varispan 4-tip Workstation Single Dispense
  • JANUS G3 Varispan 4-tip Workstation Multiple Dispense
  • Qiagen® QIAgility® Instrument
  • FlexDrop™ Non-Contact Dispenser (500 μL version)

Read the application note to review the qPCR plate prep data obtained from these different methods.

For research use only. Not for use in diagnostic procedures.

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Optimizing 384-well qPCR plate preparation while decreasing costs

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