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Technical Note

Insider tips to optimize your GPCR signaling studies

Drawing on dozens of data sets, this technical note gives you critical insights into the development, optimization, and validation of ERK phosphorylation assays.

Get the experts’ opinion on:

Cell density
Effect of serum starvation on signal
Stimulation time
Best practices in compound dispensing
Cell lysis and phosphorylation detection

For research use only. Not for use in diagnostic procedures.

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Insider tips to optimize your GPCR signaling studies

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