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Application Note

High-throughput RNA extraction from Mus musculus tissues on the Omni Bead Ruptor 12 bead mill homogenizer

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We evaluated the Omni Bead Ruptor 12 bead mill homogenizer’s ability to prepare various tissues - kidney, liver, heart, and lung - from mice for RNA extraction, compared to a traditional cryomilling method such as with mortar and pestle. Based on average RNA extraction yields, RIN values, and observance of any high molecular weight shearing, bead-beating was an effective method for tissue homogenization and lysis for RNA extraction and amenable to scaling throughput compared to manual methods.

For research use only. Not for use in diagnostic procedures.

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High-throughput RNA extraction from Mus musculus tissues on the Omni Bead Ruptor 12 bead mill homogenizer

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