High-throughput lysis of E. coli for multiple analytes using the Omni Bead Ruptor 96 bead mill homogenizer

Bacterial research used to be limited by the need to plate or grow them in culture to amplify biological analytes for proper downstream analysis and detection. With the continued advances in genomic and proteomic techniques, however, methods such as qPCR and mass spectrophotometry has positively impacted the applicability of bacterial work within various applications in basic research and pre-clinical drug discovery. To help meet the speed at which analysis is now possible, labs can now leverage bead mill technologies in a 96-well plate format to support high-throughput processing. The Omni Bead Ruptor 96 homogenizer, in conjunction with 96-well plates pre-filled with 0.1 mm ceramic bead media, was evaluated for its efficiency in lysing E. coli and recovery of DNA, RNA, and proteins. The results showed high integrity and concentration of these analytes, suggesting a highly efficient system that can stand alone or be integrated with other high-throughput sample prep workflows for various downstream analyses.

For research use only. Not for use in diagnostic procedures.