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Generating a clonal HAP1LIG4(-) Cas9(+) cell line – an expert interview

By incorporating a stably expressed Cas9 nuclease into Revvity’s HAP1 LIG4(-) cell line, the Sanger Institute has generated a new cell line that is highly amenable to efficient CRISPR/Cas9-mediated genomic modifications.

Read our interview with Dr. Sebastian Gerety, Principal Staff Scientist in the Human Genetics Program at the Sanger Institute, in which he discusses:

  • Why they developed this new HAP1 cell line
  • How leveraging the HAP1 LIG4(-) cell line developed with Revvity will help to accelerate the Sanger Institute’s research goals
  • How he envisions this new cell line impacting scientific discovery

For research use only. Not for use in diagnostic procedures.

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Generating a clonal HAP1LIG4(-) Cas9(+) cell line – an expert interview