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AlphaScreen cAMP Detection Kit
Application Note

Functional GPCR studies using AlphaScreen cAMP detection kit

G protein-coupled receptors (GPCRs) are cell surface transmembrane receptors that activate G-proteins. GPCR activity is often assessed by measuring intracellular cAMP levels upon stimulation by agonists. Since GPCRs are important pharmaceutical drug targets, there’s a high demand for methods to detect and quantify cAMP. The ideal assay is homogenous, non-radioactive, and allows for sensitive and reproducible detection of cAMP.

The AlphaScreen™ cAMP assay is one such method. It was evaluated for its ability to detect agonist or antagonist-induced cAMP responses in cells expressing either endogenous or recombinant receptors. Read this application note to access detailed data and results on GPCR studies using AlphaScreen cAMP detection kit.

For research use only. Not for use in diagnostic procedures.

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Functional GPCR studies using AlphaScreen cAMP detection kit

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