Beadless bead mill homogenization for protein extraction from soft rat brain tissue sections

Bead mill homogenizers typically rely on vigorous shaking of tubes filled with bead material to disrupt samples. The impact between the beads and the sample reduces sample particle size and creates a homogenous mixture, making bead milling an efficient method of homogenization. Heat, however, is produced through the bead milling process because of the kinetic energy generated from the beads impacting the sample and tube walls. For some applications, such as those that are particularly heat-sensitive, labs could employ solutions to mitigate this issue as much as possible using cryo-cooling units. For some applications, the presence of the bead media within the sample may be unnecessary, such as for soft tissue homogenization. In this application note, we evaluated protein yields from rat brain tissue sections using “beadless” bead-milling homogenization on the Omni Bead Ruptor Elite bead mill homogenizer.

For research use only. Not for use in diagnostic procedures.