Menu
US
Application Note

Applicability of AlphaLISA technology to a wide spectrum of complex biological samples

applicability-of-alphalisa-technology512x288

Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology is a bead-based, no-wash alternative to traditional ELISAs. Instead of detection with an HRP substrate, the Alpha assay signal is generated by the excitation of an Eu+-coated bead that has been conjugated to the detection antibody.

Alpha technology offers a simple, straight forward workflow. No wash needed!

  • Add sample
  • Add Acceptor bead mix; incubate 1 hr
  • Add Donor beads; incubate 30 mins
  • Read on Alpha-enable microplate reader

Download this application note to see how no-wash AlphaLISA™ technology provides a more-convenient alternative to ELISA for quantitation of biomarkers in complex sample types, including tissue, serum, and plasma.

For research use only. Not for use in diagnostic procedures.

To view the full content please answer a few questions

By submitting my personal data, I acknowledge that Revvity Inc. and its affiliates (“Company”) will process my personal data provided above consistent with the Company’s Privacy Policy available here.

This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Download Resource

Applicability of AlphaLISA technology to a wide spectrum of complex biological samples

Download Application Note