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Application Note

Alpha technology: a fast and sensitive orthogonal approach to cell-based potency assays

Orthogonal systems to cell-based assays are a key requirement in EMA/FDA guidelines for potency estimations and require cross-validation with complementary approaches to prove and strengthen the reliability of results.

In this application note published in collaboration with IBR Inc., you will learn:

Why Alpha technology represents an ideal cell-free orthogonal system for potency assays
How AlphaLISA assays can be used to determine Bevacizumab/VEGF165 potency
An example of how to run an AlphaLISA potency assay and the type of data that can be generated
Why it is suitable for assessing lot-to-lot consistency and equivalence of Bevacizumab and biosimilars

For research use only. Not for use in diagnostic procedures.

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Alpha technology: a fast and sensitive orthogonal approach to cell-based potency assays

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