Yeast Viability and Vitality Assay Kits and Reagents

Reagents and kits for determining yeast viability, concentration and vitality in biofuel production and brewing

Determining yeast viability and concentration during biofuel and brewing production is essential for process optimization and monitoring of alcohol production. Many of these complex yeast samples are difficult to count automatically using the brightfield method manually due to debris in the sample. By using a combination of fluorescent dyes - acridine orange (AO) and propidium iodide (PI) - Cellometer™ cell counters can more accurately determine the concentration and viability of these complex samples. The ViaStain™ CFDA-AM Yeast Vitality Stain supports the user with quantitatively distinguishing metabolically active lager and ale yeast in both pure cultures and cultures containing debris such as beer slurries using Cellometer cell counters. The percentage of yeasts that are actively fermenting during production can be determined and used to optimize the fermentation process during beer brewing, for example.