qPCR

This video provides an overview of Revvity's reproductive health offerings, designed for laboratories.

"Residual HEK293 DNA is a critical impurity that must be minimized when generating recombinant AAV (rAAV) therapeutic products, as elevated levels can pose safety risks related to immunogenic and oncogenic effects. Residual host cell DNA quantification typically requires prior sample extraction to account for interference from complex biological matrices, including variations in buffer composition, residual proteins, and ionic strength. While extraction-based, off-line testing is adequate for final drug product testing, it limits real-time, in-process quality monitoring and optimization. This application note delves into the development of the HostDetect™ HEK293 PCR DNA Quant Kit, showing how it has been designed to meet or exceed regulatory guidance by offering a PCR-based approach for quantifying residual host cell DNA. For HEK293, the high performing system is shown to deliver: - Accelerated speed ( 2hrs) - Greater sensitivity (detection as low as 0.03 pg/reaction) - Higher precision (CV 5% at 0.1 pg/reaction). Also we provide guidelines for a streamlined, sample-extraction-free approach for residual DNA quantification using the HostDetect kit. A method that offers a timesaving, efficient, and practical solution to monitor residual HEK293 DNA during rAAV manufacturing, thereby facilitating GMP process optimization."

This flyer provides an overview of the benefits offered by Revvity's Mpox Virus Real-Time PCR Kit.

Helping you put safety and product efficacy at the forefront of your GMP manufacturing At Revvity we’re able to support your GMP manufacturing workflows in areas such as detecting and quantifying harmful impurities along with helping you produce products that have both improved reliability and reproducibility. In this infographic you’ll get to grips with the instruments, reagents and assays that can help you produce safer and more efficient biotherapeutics. Covering areas of: Cell line development Screening and characterization Detection and quantification Meeting compliance requirements With it all backed by technical expertise and support, you’re biotherapeutic manufacturing just got a lot safer. For research use only. Not for use in diagnostic procedures.