High Content Screening Instruments
High Content Screening (HCS) and High Content Analysis (HCA) products
High-Content Analysis (HCA) or High-Content Screening (HCS) combines high-throughput automated imaging and analysis to extract quantitative multi-parametric data at the single-cell level. Originally developed as a complementary technology to traditional biochemical high-throughput screening (HTS) in drug discovery, today high-content screening is established in a far broader area of the life science space as an unbiased imaging method to assess cellular function.
High-Content Imaging (HCI) is typically defined as lower throughput automated microscopy (<100,000 samples or data points) compared to high-content screening. However, high-content imaging is often used interchangeably with the terms high-content screening and high-content analysis.
High-content screening offers a number of advantages over traditional techniques:
- More information from cellular samples - Fully automated imaging and unbiased quantitative image analysis exploits the full potential of microscopy, improving your lab’s throughput and productivity.
- More meaningful results - Screening can be performed in more physiologically and biologically relevant systems than in classical biochemical assays.
- More questions answered simultaneously - Data sets are more information-rich compared to non-imaging read-outs, allowing a number of questions to be answered simultaneously.
(1) Buchser, William, et al. "Assay development guidelines for image-based high content screening, high content analysis and high content imaging." Assay guidance manual (2014).
For research use only. Not for use in diagnostic procedures.
Explore our high-content screening system technologies
Confocal imaging
Spinning disk confocal microscopy offers several advantages over conventional optical microscopy, including widefield and laser scanning confocal microscopy.
Water immersion objectives
Proprietary automated water-immersion objectives with very high numerical aperture deliver and capture more photons and provide a higher resolution in XYZ than conventional objectives.
Illumination
Stability, brightness and spectral fit for excitation are key considerations for illumination sources in fluorescent imaging.
sCMOS cameras
The large-format sCMOS camera – one in Operetta CLS™ or up to four of them in the Opera Phenix - delivers low noise, wide dynamic range, and high resolution – perfect for sensitive and quantitative measurements at short exposure times.
Intelligent image acquisition
More accurately target your object of interest for significantly reduced acquisition and analysis times with intelligent acquisition technology for HCS.
Machine learning
Machine learning techniques enable supervised and unsupervised feature selection and clustering of highly multi-parametric data sets.
Explore our high-content screening system products
Operetta CLS high content analysis system
Operetta CLS™ is a high throughput microplate imager for High-Content Analysis (HCA). It can acquire, analyze and manage fluorescence, brightfield and digital phase contrast images.
Operetta CLS™ is a high throughput microplate imager for High-Content Analysis (HCA). It can acquire, analyze and manage fluorescence, brightfield and digital phase contrast images.
Opera Phenix Plus high-content imaging system - single camera
The single camera Opera Phenix Plus System is configured for the research and budget of biopharma start-ups and smaller academic labs.
The single camera Opera Phenix Plus System is configured for the research and budget of biopharma start-ups and smaller academic labs.
Opera Phenix Plus high-content screening system
The Opera Phenix™ Plus high-content screening system is the premier confocal solution for today's most demanding high content applications.
The Opera Phenix™ Plus high-content screening system is the premier confocal solution for today's most demanding high content applications.
High-content screening system filters and lenses
Enable sensitive imaging on our cellular vivo imaging systems - at whatever resolution you desire. With our custom-designed optics, including optical filters, objectives and zoom lenses, you have the ideal view for any application.
Enable sensitive imaging on our cellular vivo imaging systems - at whatever resolution you desire. With our custom-designed optics, including optical filters, objectives and zoom lenses, you have the ideal view for any application.
Meet our users
Read how scientists around the globe are using our high-content screening products and services to advance their research in a variety of fields.
Read how scientists around the globe are using our high-content screening products and services to advance their research in a variety of fields.
