CRISPR knockout
Algorithm designed CRISPR guide RNAs for highly accurate and efficient functional gene knockout.
Our guide RNAs are designed using a validated algorithm to achieve functional gene knockout with high specificity. By assessing phenotypes for thousands of designs, then validating our design rules in multiple assay systems, we have established standards for determining optimal knockout target sites.
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CRISPR knock-in
CRISPR guide RNAs program Cas9 nuclease to cut genomic DNA at a specific location. Once the double-strand break (DSB) occurs, the mammalian cell utilizes endogenous mechanisms to repair the DSB. In the presence of a donor DNA, either a ssDNA oligo or a plasmid donor, the DSB can be repaired precisely using HDR resulting in a desired genomic alteration (insertion, removal, or replacement).
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CRISPR interference
CRISPR interference (CRISPRi) allows researchers to down-regulate specific gene function by blocking transcription, without editing the DNA.
The Revvity Dharmacon CRISPRmod™ CRISPRi system requires two components to operate: a gene-specific CRISPRi guide RNA and a catalytically deactivated Cas9 (dCas9) fused to transcriptional repressor domains (SALL1 and SDS3). Our CRISPRi reagents provide a straightforward, efficient set of tools to study a gene’s function via transcriptional repression.
Featured products:
- CRISPRi synthetic sgRNA
- CRISPRi lentiviral sgRNA
- CRISPRi all-in-one lentiviral sgRNA
- dCas9-SALL1-SDS3 mRNA
- dCas9-SALL1-SDS3 lentiviral particles
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CRISPR activation
CRISPR activation (CRISPRa) allows researchers to up-regulate specific gene function by activating transcription, without editing the DNA.
The Revvity Dharmacon CRISPRmod CRISPRa system requires two components to operate: a CRISPRa guide RNA and a catalytically deactivated Cas9 (dCas9) fused to transcriptional activators (VPR). Our CRISPRa reagents provide a straightforward, efficient set of tools to study a gene’s function by overexpression in its native context.
Featured products:
- CRISPRa lentiviral sgRNA
- CRISPRa synthetic crRNA
- CRISPRa all-in-one lentiviral sgRNA
- dCas9-VPR stable cell lines
- dCas9-VPR mRNA
- dCas9-VPR lentiviral reagents
- MS2 tracrRNA
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Pin-point™ base editing reagents
Revvity's Pin-point base editing technology is a three-component system featuring a nickase Cas9 which recruits the effector deaminase through fusion to an aptamer-binding protein. The aptamer is linked to the guide RNA associated with the nuclease in an extended, chimeric RNA scaffold. The modular nature of effector recruitment means that nCas9 can be used for the knockout of one or more gene targets while simultaneously inserting a transgene.
Featured products:
- Pin-point base editing mRNA
- Pin-point synthetic sgRNA non-targeting controls
- Pin-point synthetic sgRNA validated controls
- Custom Pin-point synthetic sgRNAs
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Gene editing libraries
Achieve high functionality and superior specificity in your pooled and arrayed screens using algorithm-optimized guide RNA designs. Dharmacon synthetic sgRNA arrayed, crRNA arrayed, and lentiviral sgRNA pooled or lentiviral sgRNA arrayed for high-throughput gene editing studies.
Investigate entire gene families or biological pathways with our broad suite of custom and pre-defined libraries of Dharmacon Edit-R CRISPR-Cas9 reagents.
Featured products:
- Dharmacon Edit-R synthetic sgRNA libraries
- Cherry-pick libraries
- CRISPR tracrRNA The trans-activating CRISPR RNA (tracrRNA) is a chemically synthesized and HPLC-purified long RNA required for use with synthetic crRNA to form the complex that programs Cas9 nuclease
- Dharmacon Edit-R arrayed lentiviral sgRNA libraries
- Cherry-pick lentiviral sgRNA libraries
- Dharmacon Edit-R pooled lentiviral sgRNA libraries
- Custom Edit-R pooled lentiviral sgRNA libraries Customize your high-titer pooled screening libraries of predesigned lentiviral sgRNA for knockout studies