High throughput homogenization and DNA extraction from staple crops

Plants are essential to our diet and ecosystem; researchers are constantly working to better understand their genetic makeup and diversity.

Traditional enzymatic lysis methods to extract plant DNA are time-consuming and can be inconsistent due to the toughness of their cell walls. Physical disruption via bead mill homogenization can be an efficient alternative to both rudimentary enzymatic and manual methods. We review the workflow and determine yields from staple crops such as corn, rice, and wheat in this application note.

Automation-enabled solutions help scale throughput while providing consistent and efficient sample preparation and nucleic acid extraction from plant material. Access the full application note for the experimental setup and instrument parameters, along with yield and analyte integrity ratios for all three sample types.

For research use only. Not for use in diagnostic procedures.