Positive control lysate for AlphaLISA™ SureFire® Ultra™ Multiplex Phospho-STAT1 (Ser727)/Total STAT1 Detection Kit.
Feature | Specification |
---|---|
Application | Protein Quantification |
Positive control lysate for AlphaLISA™ SureFire® Ultra™ Multiplex Phospho-STAT1 (Ser727)/Total STAT1 Detection Kit.
The positive control lysate is prepared from HeLa cells, cultured to confluence in T175 flasks in 10% FBS containing medium, then treated consecutively with 0.6 M Sorbitol and 200 ng/mL Interferon-gamma for 20 minutes (independent treatments) and lysed in 6 mL of 1X SureFire Ultra Lysis buffer.
Application |
Protein Quantification
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra Multiplex
|
Detection Modality |
Alpha
|
Lysis Buffer Compatibility |
Lysis Buffer
|
Molecular Modification |
Phosphorylation
|
Product Group |
Control Lysate
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
STAT1
|
Target Class |
Phosphoproteins
|
Target Species |
Human
|
Technology |
Alpha
|
Unit Size |
1 Vial
|
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