AlphaLISA SureFire Ultra Human Total TIE2 Detection Kit, 500 Assay Points

The TEK receptor tyrosine kinase (TIE2), encoded by the TEK gene in humans, is primarily expressed on endothelial cells. Upon binding with angiopoietin-1 (Angpt1), TIE2 induces angiogenesis and supports endothelial cell survival. This activation leads to downstream signaling pathways, including MEK/ERK, IKKb/NFkB, and AKT. Consequently, TIE2 contributes to vascular stability, cellular proliferation, inflammation, and cell survival. Elevated levels of Ang1 and/or Ang2 have been observed in patients with various cancers.

The AlphaLISA SureFire Ultra Human Total TIE2 Detection Kit is a sandwich immunoassay for the quantitative detection of total TIE2 in cellular lysates, using Alpha technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput drug screening