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Application Note
Simultaneous detection of drug efficacy and toxicity by combining HTRF, AlphaLISA, or AlphaLISA SureFire Ultra with ATPlite
This application note demonstrates how compound’s mechanism of action and potential cytotoxic effects can be deciphered thanks to the combination of AlphaLISA™, HTRF™ or AlphaLISA™ SureFire® Ultra™ immunoassays with ATPlite™ 1step cell viability assay.
Guide
Antibody conjugation to AlphaLISA Acceptor beads detailed protocol
Detailed protocol step-by-step: recommended reagents and materials, antibody assessment and pre-treatment, bead conjugation reaction and bead coupling optimization.
Application Note
Utilization of AlphaLISA technology to accurately detect asthma biomarkers in human serum
In this application note, AlphaLISA technology was used to quantify biomarker levels in serum samples, specifically Human IL-21, IL-33, and TNFα.
Application Note
Fc-Receptor binding ADCC assays utilizing AlphaLISA technology: Characterization of hIgGs and FcγRIIIa
This application note demonstrates how AlphaLISA assay can be used to determine the binding activity of human IgG Fc fragment to human FcγRIIIa and to study how other antibodies bind to FcγRIIIa.
Application Note
Tracking inflammasome activity via measuring IL-1β levels with AlphaLISA and HTRF
This application note demonstrates the utility of AlphaLISA™ and HTRF™ for the detection of IL-1β in cell supernatant and serum samples which can be used as a biomarker for inflammasome activity
Whitepaper
Exploring current and future approaches for targeting KRAS
Get the latest insights on KRAS and see how it has switched from being an undruggable to a druggable cancer target
Application Note
No-Wash IP1 assays are a powerful readout to characterize compounds modulating the FGFR signaling pathway in cancer drug research
This application note demonstrates that detecting the intracellular accumulation of IP1, mediated by FGFR-dependent activation of PLCγ1, can be a powerful alternative for characterizing compounds that modulate FGFR signaling in various cancer cell lines expressing FGFR1, FGFR2, and FGFR3.
Application Note
cAMP AlphaScreen assay: a method for the pharmacological characterization and screening of Gαi coupled receptors in whole cells
The note demonstrates the utility of the AlphaScreen-based cAMP Assay kit for the pharmacological characterization and potential high-throughput screening of Gαi-coupled receptors.
Product Info
LANCE and LANCE Ultra TR-FRET technology product list
LANCE™ Ultra is a highly sensitive and highly reproducible no-wash proximity assay technology that combines the benefits of time resolution (TR) with fluorescence resonance energy transfer (FRET).
Flyer
Bioanalytical methods for biosimilarity assessment
Working in partnership to develop and qualify assays in the support of the biosimilarity assessment data package.
Application Note
Guidelines for optimization of a cell-labelling method for DELFIA-TRF cytotoxicity assays.
Discover expert guidelines for optimizing cell labeling in DELFIA TRF cytotoxicity assays. Learn how to enhance assay performance, manage spontaneous release, and achieve accurate results with this comprehensive application note.
Application Note
Simultaneous measurement of chemokine/cytokine release and antibody-dependent cellular cytotoxicity upon binding with rituximab
Simultaneous detection of ADCC activity and release of chemokines and cytokines from activated NK cells is possible via the combined use of DELFIA TRF and AlphaLISA technologies.
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