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NEXTFLEX Cell Free DNA-Seq Library Prep Kit 2.0
NEXTFLEX Cell Free DNA-Seq Library Prep Kit 2.0
Revvity kit box for reagents and consumables
The NEXTFLEX® Cell Free DNA-Seq Kit 2.0 can produce libraries from 10 ng of cell free DNA for circulating tumor DNA (ctDNA) or cell free fetal DNA (cffDNA) analysis, for sequencing on Illumina® or Element Biosciences® NGS platforms in two hours.
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| Feature | Specification |
|---|---|
| Automation Compatible | Yes |
| Product Group | DNA-seq |
The NEXTFLEX® Cell Free DNA-Seq Kit 2.0 can produce libraries from 10 ng of cell free DNA for circulating tumor DNA (ctDNA) or cell free fetal DNA (cffDNA) analysis, for sequencing on Illumina® or Element Biosciences® NGS platforms in two hours.
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Product Variants
Unit Size: 8 rxns
Part #:
NOVA-5150-01
List Price
USD 192.00
Your online price:
Unit Size: 48 rxns
Part #:
NOVA-5150-02
List Price
USD 1,152.00
Your online price:
For research use only. Not for use in diagnostic procedures.
NEXTFLEX Cell Free DNA-Seq Library Prep Kit 2.0
Revvity kit box for reagents and consumables
NEXTFLEX Cell Free DNA-Seq Library Prep Kit 2.0
Supporting products you might need
Do you need Unique Dual Index Barcodes?
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Part #: NOVA-514150-EVAL48
List Price: USD 489.60
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Product information
Overview
- Designed for low sample input – 10 ng of input cfDNA required
- Accelerated workflow with minimized hands-on time
-
Flexible adapter barcode options:
- Up to 1,536 unique dual index barcodes are available.
- Up to 96 UDI-UMI Barcodes now available to improve the detection of rare variants.
- Compatible with Illumina® and Element Biosciences® sequencing platforms
- Protocols available for automation on the Sciclone® G3 NGSx, NGSx iQ™ and Zephyr® G3 NGS workstations
The NEXTFLEX® Cell Free DNA-Seq Kit 2.0 delivers high coverage and reduced bias for Illumina® sequencing applications. The NEXTFLEX® Cell Free DNA-Seq Kit 2.0 can be used to prepare single, paired-end and multiplexed DNA libraries for sequencing using Illumina® platforms. The availability of up to 1,536 unique dual index barcodes facilitates high-throughput applications without compromising data integrity.
Reduced Bias and Coverage Gaps
NEXTFLEX® Cell Free DNA-Seq Kit 2.0 exhibits minimal GC bias and produces uniform coverage of difficult to sequence genome regions. This kit also offers robust ligation efficiency, resulting in greater library diversity and a larger number of unique sequencing reads. These optimizations enable investigators to obtain more information from each sequencing run.
Flexible Multiplexing Options
The NEXTFLEX® Cell Free DNA-Seq Library Prep Kit 2.0 is designed to be used with the NEXTFLEX® Unique Dual Index barcodes (384 barcodes) or the NEXTFLEX® Unique Dual Index barcodes (1,536 barcodes). NEXTFLEX UDI-UMI adapters, incorporating molecular barcodes into each adapter, are also available for applications involving the detection of rare variants.
Specifications
| Automation Compatible |
Yes
|
|---|---|
| Product Group |
DNA-seq
|
| Shipping Conditions |
Dual Temperature
|
| Unit Size |
48 rxns
|
Resources
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