Overview
|
Reagent |
Suggested source |
Catalog number |
|---|---|---|
|
Kinases |
Various suppliers (Carna, Millipore, ProQinase) |
|
|
HEPES |
Sigma-Aldrich |
H3375 |
|
ATP |
Sigma-Aldrich |
A7699 |
|
DTT |
Sigma-Aldrich |
D0632 |
|
EGTA |
Sigma-Aldrich |
E4378 |
|
MgCl2 |
Sigma-Aldrich |
M9272 |
|
EDTA |
Invitrogen |
15575-020 |
|
Tween-20 |
Pierce |
28320 |
|
Ultra-Pure water (18 mega ohms/cm) |
various suppliers |
|