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LANCE Citations

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LANCE Citations
LANCE ULight dye spectra
LANCE Citations
LANCE Indirect 4-Component Kinase Assays
LANCE Classic 3- Kinase Assay
LANCE Epigenetic Assays
Suppliers for other cAMP reagents
Suppliers for other kinase assay reagents
Label Your Own LANCE Reagent
LANCE Products and Catalog Numbers
APC dye spectra
LANCE Eu-W8044-DTA spectra
LANCE Eu-W1024-ITC spectra
LANCE Instrument Options and Settings
LANCE kinase assays optimization
LANCE Ultra cAMP Assays
LANCE Ultra Kinase Assays
LANCE Protein-Protein Interaction Assays
LANCE and LANCE Ultra TR-FRET Assays
LANCE Phosphatase Assays

Overview

On this page we provide selected citations for each application.

LANCE cAMP assays

Journal articles

  1. Ben-Shlomo, A. et al. Constitutive Somatostatin Receptor Activity Determines Tonic Pituitary Cell Response. Mol Endocrinol 23, 337-348 (2009).  Link
  2. Chen, C., McLachlan, S.M. & Rapoport, B. Identification of Key Amino Acid Residues in a Thyrotropin Receptor Monoclonal Antibody Epie Provides Insight into Its Inverse Agonist and Antagonist Properties. Endocrinology 149, 3427-3434 (2008).  Link
  3. Doucette, C. et al. Kappa Opioid Receptor Screen with the Tango Beta-Arrestin Recruitment Technology and Characterization of Hits with Second-Messenger Assays. J Biomol Screen 14, 381-394 (2009).  Link
  4. Hamilton, B.S. & Doods, H.N. Identification of potent agonists acting at an endogenous atypical Beta3-adrenoceptor state that modulate lipolysis in rodent fat cells. Eur J Pharmacol 580, 55-62 (2008).  Link
  5. Hendriks-Balk, M.C. et al. S1P Receptor Signalling and RGS Proteins; Expression and Function in Vascular Smooth Muscle Cells and Transfected CHO Cells. Eur J Pharmacol 600, 1-9 (2008).  Link
  6. Knight, J.A., Smith, C., Toohey, N., Klein, M.T. & Teitler, M. Pharmacological Analysis of the Novel, Rapid, and Potent Inactivation of the Human 5-Hydroxytryptamine7 Receptor by Risperidone, 9-OH-Risperidone, and Other Inactivating Antagonists. Mol Pharmacol 75, 374-380 (2009).  Link
  7. Mizutori, Y., Chen, C., Latrofa, F., McLachlan, S.M. & Rapoport, B. Evidence that Shed Thyrotropin Receptor A Subunits Drive Affinity Maturation of Autoantibodies Causing Graves' Disease. J Clin Endocrinol Metab 94, 927-935 (2009).  Link
  8. Xia, M. et al. Identification of Compounds that Potentiate CREB Signaling as Possible Enhancers of Long-Term Memory. P Natl Acad Sci USA 106, 2412-2417 (2009).  Link

LANCE Ultra cAMP journal articles

  1. Townsend, E. A., Sathish, V., Thompson, M. A., Pabelick, C. M. & Prakash, Y. S. Estrogen effects on human airway smooth muscle involve cAMP and protein kinase A. Am. J. Physiol. Lung Cell Mol. Physiol. 303, L923–928 (2012).
  2. Blättermann, S. et al. A biased ligand for OXE-R uncouples Gα and Gβγ signaling within a heterotrimer. Nat. Chem. Biol. 8, 631–638 (2012).
  3. Oddi, S. et al. Effects of palmitoylation of Cys(415) in helix 8 of the CB(1) cannabinoid receptor on membrane localization and signalling. Br. J. Pharmacol. 165, 2635–2651 (2012).
  4. Li, Y. et al. Identification of two functionally distinct endosomal recycling pathways for dopamine D₂ receptor. J. Neurosci. 32, 7178–7190 (2012).
  5. Nikas, S. P. et al. Novel 1’,1’-chain substituted hexahydrocannabinols: 9β-hydroxy-3-(1-hexyl-cyclobut-1-yl)-hexahydrocannabinol (AM2389) a highly potent cannabinoid receptor 1 (CB1) agonist. J. Med. Chem. 53, 6996–7010 (2010).
  6. Schröder, R. et al. Applying label-free dynamic mass redistribution technology to frame signaling of G protein-coupled receptors noninvasively in living cells. Nat Protoc 6, 1748–1760 (2011).

Revvity technical publications

  1. Hurt, S. et al. Automation of a GPCR Cell-based Functional Assay on the Cellular Workstation System using the LANCE cAMP Technology. Revvity Poster.
  2. Kasila, P. and Harney, H. LANCE cAMP - Increased Sensitivity, Stability, and Overall Assay Performance with Gαs and Gαi coupled receptors in a miniaturized 1536-well format. Revvity Poster.
  3. Kasila, P. and Xie, H. LANCE cAMP - A uHTS cAMP detection assay for use with membranes. Revvity Poster.
  4. Kasila, P., Warner, G., Stuchly, C., Evans, K.L. & Fletcher, P. A Phosphodiesterase Assay using LANCE Technology: Simple, Flexible and Sensitive. Revvity Poster.

LANCE biomolecular interaction assays

Journal articles

  1. Mitro, N., Godio, C. & Crestani, M. Fluorescence Resonance Energy Transfer Techniques to Study Ligand-Mediated Interactions of PPARs with Coregulators. Methods Mol. Biol. 952, 219–227 (2013).
  2. Dams, G. et al. A Time-Resolved Fluorescence Assay to Identify Small-Molecule Inhibitors of HIV-1 Fusion. J Biomol Screen 12, 865-874 (2007).  Link
  3. Lindqvist, L. et al. Selective Pharmacological Targeting of a DEAD Box RNA Helicase. PLoS ONE 3, e1583 (2008). Link
  4. Mehle, A. et al. Identification of an APOBEC3G Binding Site in Human Immunodeficiency Virus Type 1 Vif and Inhibitors of Vif-APOBEC3G Binding. J Virol 81, 13235-13241(2007). Link
  5. Najima, Y. et al. High Mobility Group Protein-B1 Interacts with Sterol Regulatory Element-binding Proteins to Enhance Their DNA Binding. J Biol Chem 280, 27523-27532 (2005). Link
  6. Nakamura, K. et al. Homogeneous Time-Resolved Fluorescence Resonance Energy Transfer Assay for Measurement of Phox/Bem1p (PB1) Domain Heterodimerization. J Biomol Screen 13, 396-405 (2008). Link
  7. Porter, J. et al. Tetrahydroisoquinoline Amide Substituted Phenyl Pyrazoles as Selective Bcl-2 Inhibitors. Bioorg Med Chem Lett 19, 230-233 (2009). Link
  8. Zhou, V. et al. A Time-Resolved Fluorescence Resonance Energy Transfer-Based HTS Assay and a Surface Plasmon Resonance-Based Binding Assay for Heat Shock Protein 90 Inhibitors. Anal Biochem 331, 349-357 (2004). Link

Revvity technical publications

  1. Hurskainen, P., Frang, H., Vikstrom, S., Karvinen, J. & Hemmilä, I. Heterogeneous and Homogeneous Time-resolved Fluorescence-based Assays for a Low-Affinity Binding Reaction. Revvity Poster

LANCE nuclear receptor assays

Journal articles

  1. Glickman, J.F. et al. A Comparison of ALPHAScreen, TR-FRET, and TRF as assay methods for FXR nuclear receptors. J Biomol Screen 7, 3-10 (2002).  Link
  2. Dietz, M. et al. Comparative molecular profiling of the PPARα/γ activator aleglitazar: PPAR selectivity, activity and interaction with cofactors. ChemMedChem 7, 1101–1111 (2012).
  3. Gunther, J.R. et al. A Set of Time-Resolved Fluorescence Resonance Energy Transfer Assays for the Discovery of Inhibitors of Estrogen Receptor-Coactivator Binding. J Biomol Screen 14, 181-193 (2009).  Link
  4. Kane, C.D. et al. Molecular Characterization of Novel and Selective Peroxisome Proliferator-Activated Receptor α Agonists with Robust Hypolipidemic Activity in Vivo. Mol Pharmacol 75, 296-306 (2009). Link
  5. Liu, J. et al. A Homogeneous in Vitro Functional Assay for Estrogen Receptors: Coactivator Recruitment. Mol Endocrinol 17, 346-355 (2003).  Link
  6. Urizar, N.L. et al. A Natural Product that Lowers Cholesterol as an Antagonist Ligand for FXR. Science 296, 1703-1706 (2002).  Link
  7. Wu, X., Sills, M.A. & Zhang, J. Further Comparison of Primary Hit Identification by Different Assay Technologies and Effects of Assay Measurement Variability. J Biomol Screen 10, 581-589 (2005). Link

LANCE kinase assays

Journal articles

  1. Zhu, W. et al. Synthesis and Biological Evaluation of Novel 6-Hydrazinyl-2,4-bismorpholino pyrimidine and 1,3,5-Triazine Derivatives as Potential Antitumor Agents. Archiv der Pharmazie (2012).
  2. McGhie, T. K., Martin, H. & Lunken, R. C. M. The combination of analytical-scale HPLC separation with a TR-FRET assay to investigate JAK2 inhibitory compounds in a Boysenberry drink. Food Funct (2012).
  3. Chen, S.-M. et al. Inhibition of tumor cell growth, proliferation and migration by X-387, a novel active-site inhibitor of mTOR. Biochemical Pharmacology (2012).Doti, N., Marasco, D., Pedone, C., Sabatella, M. & Ruvo, M. Optimizing a Kinase Assay for IKKB on an HTS Station. J Biomol Screen (2009). Link
  4. Jin, G. et al. Development and Comparison of Nonradioactive in Vitro Kinase Assays for NIMA-Related Kinase 2. Anal Biochem 358, 59-69 (2006). Link
  5. Moshinsky, D.J., Ruslim, L., Blake, R.A. & Tang, F. A Widely Applicable, High-Throughput TR-FRET Assay for the Measurement of Kinase Auhosphorylation: VEGFR-2 as a Prototype. J Biomol Screen 8, 447-452 (2003). Link
  6. Patnaik, D. et al. Identification of Small Molecule Inhibitors of the Mitotic Kinase Haspin by High-Throughput Screening Using a Homogeneous Time-Resolved Fluorescence Resonance Energy Transfer Assay. J Biomol Screen 13, 1025-1034 (2008). Link
  7. Sabbatini, P. et al. Antitumor Activity of GSK1904529A, a Small-Molecule Inhibitor of the Insulin-like Growth Factor-I Receptor Tyrosine Kinase. Clin Cancer Res 15, 3058-3067 (2009).  Link
  8. Zhang, W., Shor, B. & Yu, K. Identification and Characterization of a Constitutively T-Loop Phosphorylated and Active Recombinant S6K1: Expression, Purification, and Enzymatic Studies in a High Capacity Non-Radioactive TR-FRET LANCE Assay. Protein Expres Purif 46, 414-420 (2006).  Link

Revvity technical publications

  1. Bielefeld-Sévigny, M. et al. Homogeneous and non-radioactive Cellular Assay Platforms for the Characterization of Kinase Inhibitors. Poster by Revvity and University of Lausanne.
  2. Blouin, J. et al. Novel Substrates for Fluorescence-based Protein Tyrosine Kinase Assays. Revvity Poster.
  3. Caron, M. et al. Development and Optimization of Kinase Assays using New LANCE Ultra TR-FRET Reagents. Revvity Application Note.
  4. Legault, M., Roby, P., Beaudet, L. & Rouleau, N. Comparison of LANCE Ultra TR-FRET to Revvity's Classical LANCE TR-FRET Platform for Kinase Applications. Revvity Application Note.
  5. Legault, M. et al. Comparison of LANCE Ultra with Other TR-FRET Platforms for a Src Tyrosine Kinase Assay. Revvity Poster. Link
  6. Padros, J. et al. Development, Automation, and Miniaturization of High-Throughput Serine/Threonine Kinase Assays Using the LANCE Ultra Platform. Revvity Poster.
  7. True, D., Kasila, P. & Harney, H. Comparison of Kinase Assay Technologies for High Throughput Screening. Revvity Poster (2002). 
  8. Warner, G. Measurement of p38/MAPK Activity Using LANCE. Revvity Application Note.
  9. Miniaturization of LANCE Kinase Assays. Revvity Application Note.
  10. How to Optimize a Tyrosine Kinase Assay Using Time Resolved Fluorescence-Based LANCE Detection. Revvity Application Note. 

Receptor-ligand assays

Journal articles

  1. Gasser, J., Hehl, M. & Millward, T.A. A Homogeneous Time-Resolved Fluorescence Resonance Energy Transfer Assay for Phosphatidylserine Exposure on Apoptotic Cells. Anal Biochem 384, 49-55 (2009). Link
  2. Moore et al. A Homogenous 384-Well High Throughput Screen for Novel Tumor Necrosis Factor Receptor: Ligand Interactions Using Time Resolved Energy Transfer. J Biomol Screen 4, 205-214 (1999). Link
  3. Pandit, S. et al. Functional Analysis of Sites within PCSK9 Responsible for Hypercholesterolemia. J Lipid Res 49, 1333-1343 (2008). Link
  4. Stenroos, K. et al. Homogeneous Time-Resolved IL-2-IL-2R Alpha Assay using Fluorescence Resonance Energy Transfer. Cytokine 10, 495-499 (1998). Link
  5. Yang, X. et al. Binding Characterization of the Interleukin-13 Signaling Complex and Development of a Ternary Time-Resolved Fluorescence Resonance Energy Transfer Assay. Anal Biochem 376, 206-212 (2008). Link

     

Revvity technical publications

  1. Frang, H., Lahteenmaki, K., Hurskainen, P. & Hemmilä, I. Homogeneous Receptor Ligand Binding Assay Based on TR-FRET. Revvity Application Note. 
  2. Stenroos, K. et al. Homogeneous Time-Resolved Fluorescence Energy Transfer Assay (LANCE) for the Determination of IL-2/IL-2 Receptor alpha interaction. Poster by Abo Akademi University and Revvity.

Receptor dimerization assays

Journal articles

  1. Carrillo, J.J., Pediani, J. & Milligan, G. Dimers of Class A G Protein-Coupled Receptors Function via Agonist-Mediated Trans-Activation of Associated G Proteins. J Biol Chem 278, 42578-42587 (2003). Link
  2. Kong, M.M.C., Fan, T., Varghese, G., O'dowd, B.F. & George, S.R. Agonist-Induced Cell Surface Trafficking of an Intracellularly Sequestered D1 Dopamine Receptor Homo-Oligomer. Mol Pharmacol 70, 78-89 (2006). Link
  3. McVey, M. et al. Monitoring Receptor Oligomerization Using Time-Resolved Fluorescence Resonance Energy Transfer and Bioluminescence Resonance Energy Transfer. The Human Delta-Opioid Receptor Displays Constitutive Oligomerization at the Cell Surface, which is not Regulated by Receptor Occupancy. J. Biol. Chem 276, 14092-14099 (2001). Link
  4. So, C.H. et al. Calcium Signaling by Dopamine D5 Receptor and D5-D2 Receptor Hetero-Oligomers Occurs by a Mechanism Distinct from That for Dopamine D1-D2 Receptor Hetero-Oligomers. Mol Pharmacol 75, 843-854 (2009). Link

Cell-based assays

Journal articles

  1. Gasser, J., Hehl, M. & Millward, T.A. A Homogeneous Time-Resolved Fluorescence Resonance Energy Transfer Assay for Phosphatidylserine Exposure on Apoptotic Cells. Anal Biochem 384, 49-55 (2009). Link
  2. Hassan, N. et al. Development of an Insect Cell-Based Assay for Detection of Kinase Inhibition using NF-kappaB Inducing Kinase as a Paradigm. Biochem J 419, 65-73 (2008). Link
  3. Lundin, K., Blomberg, K., Nordström, T. & Lindqvist, C. Development of a Time-Resolved Fluorescence Resonance Energy Transfer Assay (Cell TR-FRET) for Protein Detection on Intact Cells. Anal Biochem 299, 92-97 (2001). Link
  4. McVey, M. et al. Monitoring Receptor Oligomerization Using Time-Resolved Fluorescence Resonance Energy Transfer and Bioluminescence Resonance Energy Transfer. The Human Delta-Opioid Receptor Displays Constitutive Oligomerization at the Cell Surface, Which is Not Regulated by Receptor Occupancy. J. Biol. Chem 276, 14092-14099 (2001). Link
  5. Stenroos, K. et al. Homogeneous Time-Resolved IL-2-IL-2R Alpha Assay Using Fluorescence Resonance Energy Transfer. Cytokine 10, 495-499 (1998). Link

Revvity technical publications

  1. Bielefeld-Sévigny, M. et al. Homogeneous and non-radioactive Cellular Assay Platforms for the Characterization of Kinase Inhibitors. Poster by Revvity and University of Lausanne.

Immunoassays/analyte detection

Journal articles

  1. Blomberg, K., Hurskainen, P. & Hemmilä, I. Terbium and Rhodamine as Labels in a Homogeneous Time-Resolved Fluorometric Energy Transfer Assay of the Beta Subunit of Human Chorionic Gonadotropin in Serum. Clin. Chem 45, 855-861 (1999). Link
  2. Kuroda, K. et al. Efficient Antibody Production upon Suppression of O Mannosylation in the Yeast Ogataea minuta. Appl. Environ. Microbiol. 74, 446-453 (2008).  Link
  3. Qin, Q., Peltola, O. & Pettersson, K. Time-resolved Fluorescence Resonance Energy Transfer Assay for Point-of-Care Testing of Urinary Albumin. Clin Chem 49, 1105-1113 (2003). Link
  4. Yu, V. et al. High capacity Homogeneous Non-Radioactive Cortisol Detection Assays for Human 11Beta-Hydroxysteroid Dehydrogenase Type 1. Assay Drug Dev Technol 5, 105-115 (2007). Link

Revvity technical publications

  1. Beaudet, L. et al. New LANCE Assays for the High Throughput Quantitation of Cytokine Biomarkers. Revvity Poster.

Ubiquitination

Journal articles

  • Boisclair, M.D. et al. Development of a Ubiquitin Transfer Assay for High Throughput Screening by Fluorescence Resonance Energy Transfer. J Biomol Screen 5, 319-328 (2000). Link
  • Hong, C.A. et al. Development of a High Throughput Time-Resolved Fluorescence Resonance Energy Transfer Assay for TRAF6 Ubiquitin Polymerization. Assay Drug Dev Technol 1, 175-180 (2003). Link
  • Murray, M.F. et al. A High-Throughput Screen Measuring Ubiquitination of p53 by Human mdm2. J Biomol Screen 12, 1050-1058 (2007). Link

     

     

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