Overview
IVISense™ Transferrin Receptor 750 (formerly Transferrin-Vivo) is a near infrared (NIR) targeted fluorescent imaging probe, where recombinant transferrin is conjugated to IVISense Fluorescent Dye (formerly VivoTag), designed to bind to transferrin receptors (TfRs) expressed in cancer cells. Because of rapid cell division and stringent demand for iron (for heme synthesis), many cancer cell types overexpress TfRs. It has been used as a targeting probe for cancer cells. In addition, TfR accumulates in locals of inflammation due to the leakage of plasma proteins and to the expression of TfR receptors by inflammatory cells. During inflammation monocytes leave the blood and differentiate into macrophages at the extravascular tissues, macrophages express surface Tf receptors.
Products and catalog numbers
| Product | Catalog number | Ex/Em wavelength (nm) | Molecular weight (g/mol) | Validated Experiments | Applications |
|---|---|---|---|---|---|
| IVISense Transferrin Receptor 750 | NEV10091 | 750 ex/770 em | 106,000 | In vivo/ex vivo In vitro |
Oncology Inflammation |
Using IVISense Transferrin Receptor 750 probe for in vivo/ex vivo studies
View instructions on setting up an in vivo imaging mouse experiment with IVISense Transferrin Receptor 750 fluorescent probe.
| Route of Injection | Mouse Dose (25 g) | Rat Dose (250 g) | Blood t 1/2 | Tissue t 1/2 | Optimal imaging time | Optimal Re-injection Time (complete clearance) | Route of Metabolism/ background tissue | FMT IVIS settings |
|---|---|---|---|---|---|---|---|---|
| IV | 2 nmol | 6-20 nmol | 10 h | 24 h | 24 (6-24) h | 96 h | Liver, kidney | FMT 750/770 IVIS 745/800 |
Frozen Tissue Protocol
We have validated IVISense Transferrin Receptor 750 for use with frozen tissue samples. Here is a brief protocol with a recommended concentration of probe to use:
Freeze tumor (without probe) and section 5-10 µm by cryostat.
Incubate with 10 µM IVISense Transferrin Receptor 750 at RT for 1 hour.
Wash 1x with PBS.
Mount with anti-fade reagent.
Fluorescence microscopy filter: Cy5.5 or Cy7*
Using IVISense Transferrin Receptor 750 probe for in vitro studies
Flow cytometry and in vitro microscopy
We have validated IVISense Transferrin Receptor 750 for use with fluorescence microscopes and flow cytometers. Here is a brief protocol with a recommended concentration of probe to use:
Culture cells in standard TC plate or chamber slide.
Incubate cells with 1 µM IVISense Transferrin Receptor 750 for 1 hour at 37 °C.
Wash 1x with PBS. For flow cytometry, detach and resuspend cells in PBS.
Flow cytometry filter settings: 712/21
Fluorescence micrsocopy filter: Cy5.5 or Cy7
For research use only. Not for use in diagnostic procedures.
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